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PMID: 1325641 Published · ppublish English Journal Article

A region of herpes simplex virus VP16 can substitute for a transforming domain of Epstein-Barr virus nuclear protein 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 17 ·1992-09-01 ·Pages 8030-4

Cohen JI

Abstract

Epstein-Barr virus (EBV) nuclear protein 2 (EBNA-2) is essential for EBV-induced B-cell transformation in vitro. EBNA-2 contains a 14-amino acid domain that directly activates transcription and is required for transformation. To determine whether another transcriptional activator can substitute for this function, a chimeric virus was constructed that contained a portion of the transcriptional activation domain from the herpes simplex virus VP16 protein inserted in place of the 14-amino acid domain of EBNA-2. The chimeric virus was able to transform B cells efficiently and transactivate expression of EBV and B-cell genes. Randomization of the 14-amino acid sequence in the domain markedly reduced its transcriptional activating activity and the transforming efficiency of the recombinant EBV. Mutation of a tryptophan within the 14-amino acid domain of EBNA-2 completely abolished transcriptional activation and B-cell transformation. These experiments indicate that EBNA-2 and VP16 activate transcription by similar mechanisms and that transcriptional activation is required for EBV-induced B-cell transformation.

MeSH Terms
Amino Acid Sequence Antigens, Viral/chemistry Cell Transformation, Viral Cells, Cultured DNA-Binding Proteins/chemistry Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human/genetics Humans In Vitro Techniques Molecular Sequence Data Recombinant Fusion Proteins Simplexvirus/genetics Structure-Activity Relationship Transcription Factors/chemistry Transcription, Genetic
Chemicals
Antigens, Viral DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens Recombinant Fusion Proteins Transcription Factors
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cohen J I
Laboratory of Clinical Investigation, National Institutes of Health, Bethesda, MD 20892.
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.17.8030
Published
1992-09-01
Pages
8030-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49849
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.17.8030 PMC Full Text View journal details
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