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PMID: 1328254 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Inhibition of actin filament depolymerization by the Dictyostelium 30,000-D actin-bundling protein.

The Journal of cell biology ·Vol. 119 ·No. 3 ·1992-11-00 ·Pages 559-67

Zigmond SH, Furukawa R, Fechheimer M

Abstract

We have studied the effect of the Dictyostelium discoideum 30,000-D actin-bundling protein on the assembly and disassembly of pyrenyl-labeled actin in vitro. The results indicate that the protein is a potent inhibitor of the rate of actin depolymerization. The inhibition is rapid, dose dependent, and is observed at both ends of the filament. There is little effect of 30-kD protein on the initial rate of elongation from F-actin seeds or on the spontaneous nucleation of actin polymerization. We could detect little or no effect on the critical concentration. The novel feature of these results is that the filament ends are free for assembly but are significantly impaired in disassembly with little change in the critical concentration at steady state. The effects appear to be largely independent of the cross-linking of actin filaments by the 30-kD protein. Actin cross-linking proteins may not only cross-link actin filaments, but may also differentially protect filaments in cells from disassembly and promote the formation of localized filament arrays with enhanced stability.

MeSH Terms
Actins/metabolism Animals Calcium-Binding Proteins/isolation & purification,metabolism Carrier Proteins/isolation & purification,metabolism Dictyostelium/metabolism Gelsolin Kinetics Macromolecular Substances Microfilament Proteins/isolation & purification,metabolism Molecular Weight Time Factors
Chemicals
Actins Calcium-Binding Proteins Carrier Proteins F-actin-binding proteins Gelsolin Macromolecular Substances Microfilament Proteins actin filament bundling proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zigmond S H
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Furukawa R
Fechheimer M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.3.559
Published
1992-11-00
Pages
559-67
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289678
Subset
IM
Grants
NIAID NIH HHS · AI 19883 · United States
External Links
PubMed source DOI: 10.1083/jcb.119.3.559 PMC Full Text View journal details
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