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PMID: 1329033 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Disulfide bonds are required for Serratia marcescens nuclease activity.

Nucleic acids research ·Vol. 20 ·No. 19 ·1992-10-11 ·Pages 4971-4

Ball TK, Suh Y, Benedik MJ

Abstract

The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

MeSH Terms
Alleles Blotting, Western Cloning, Molecular Cysteine Disulfides Endodeoxyribonucleases Endonucleases/genetics,isolation & purification,metabolism Endoribonucleases Genes, Bacterial Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Plasmids Recombinant Proteins/isolation & purification,metabolism Serratia marcescens/enzymology,genetics
Chemicals
Disulfides Recombinant Proteins Endodeoxyribonucleases Endonucleases Endoribonucleases Serratia marcescens nuclease Cysteine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ball T K
Department of Biochemical and Biophysical Sciences, University of Houston, TX 77204-5934.
Suh Y
Benedik M J
References (15)
15 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.19.4971
Published
1992-10-11
Pages
4971-4
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334271
Subset
IM
Grants
NIGMS NIH HHS · GM36891 · United States
Databases
GENBANK
M19495
External Links
PubMed source DOI: 10.1093/nar/20.19.4971 PMC Full Text View journal details
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