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PMID: 1335406 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Agents capable of eliminating reactive oxygen species. Catalase, WR-2721, or Cu(II)2(3,5-DIPS)4 decrease experimental colitis.

Digestive diseases and sciences ·Vol. 37 ·No. 12 ·1992-12-00 ·Pages 1866-73

Keshavarzian A, Haydek J, Zabihi R, Doria M, D'Astice M, Sorenson JR

Abstract

Reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide, hydroxyl radical, and hypochlorous acid have been implicated in the pathogenesis of inflammation and tissue injury in colitis. To determine whether or not anti-ROS agents can decrease the severity of colitis, we evaluated the effects of three known anti-ROS agents: catalase, WR-2721, and Cu(II)2(3,5-DIPS)4 on acetic acid-induced colonic inflammation in rats. Histologically, all three compounds significantly decreased the severity of colonic inflammation. The anti-ROS activity of these compounds was also tested using the luminol-enhanced chemiluminescence assay. Catalase, WR-2721, or Cu(II)2(3,5-DIPS)4 significantly inhibited luminol-enhanced chemiluminescence produced by inflamed colonic mucosa. These findings suggest that ROS, and in particular superoxide, hydrogen peroxide, and/or one of its secondarily derived species, may play an important role in acetic acid-induced colitis. Further studies are needed to determine the potential effectiveness of these compounds in human colitis.

MeSH Terms
Acetates Acetic Acid Amifostine/pharmacology Animals Catalase/pharmacology Colitis/chemically induced,metabolism,pathology Female Luminescent Measurements Rats Rats, Inbred F344 Reactive Oxygen Species/metabolism Salicylates/pharmacology
Chemicals
Acetates Reactive Oxygen Species Salicylates copper bis(3,5-diisopropylsalicylate) Catalase Amifostine Acetic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Keshavarzian A
Department of Medicine, Loyola University Medical School, Maywood, Illinois.
Haydek J
Zabihi R
Doria M
D'Astice M
Sorenson J R
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Article Info
Journal
Digestive diseases and sciences
Abbr.
Dig Dis Sci
ISSN
0163-2116
DOI
10.1007/BF01308081
Published
1992-12-00
Pages
1866-73
Language
English
Region
United States
NLM ID
7902782
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01308081 View journal details
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