The preparation, purification, and amino acid sequence of a polypeptide renin substrate.

The Journal of experimental medicine ·Vol. 106 ·No. 3 ·1957-09-01 ·Pages 439-53

SKEGGS LT, KAHN JR, LENTZ K, SHUMWAY NP

Abstract

A purified preparation of a polypeptide renin substrate prepared by tryptic degradation of the protein renin substrate has been analyzed by the fluorodinitrobenzene method and after degradation with renin, carboxypeptidase, and phenylisothiocyanate, has been found to possess the amino acid sequence; asp-arg-val-tyr-ileu-his-pro-phe-his-leu-leu-val-tyr-ser. The first 10 of these amino acids constitutes hypertensin I which is released by cleavage of the leucyl-leucine bond by renin. The remaining 4 amino acids, leu, val, tyr, ser, apparently link hypertensin I to the protein renin substrate.

Keywords

PEPTIDES PROTEASES

MeSH Terms

Amino Acid Sequence Amino Acids Angiotensinogen Dipeptides Endopeptidases Peptide Hydrolases Peptides

Chemicals

Amino Acids Dipeptides Peptides arginylvaline valyltyrosine Angiotensinogen histidylproline leucylleucine histidylleucine Endopeptidases Peptide Hydrolases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

SKEGGS L T

KAHN J R

LENTZ K

SHUMWAY N P

References (16)

16 references, click to expand

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J Exp Med. 1952 Mar;95(3):241-6 PMID: 14927788
The purification of hypertensin II.
J Exp Med. 1956 Mar 1;103(3):301-7 PMID: 13295488
Amino acid composition and electrophoretic properties of hypertensin I.
J Exp Med. 1955 Oct 1;102(4):435-40 PMID: 13263485
Recent developments in techniques for terminal and sequence studies in peptides and proteins.
Methods Biochem Anal. 1955;2:359-425 PMID: 14393573
Artificial kidney; construction and operation of an improved continuous dialyzer.
Proc Soc Exp Biol Med. 1949 Dec;72(3):539-43 PMID: 15400789
The assay of hypertensin from the arterial blood of normotensive and hypertensive human beings.
J Exp Med. 1952 Jun;95(6):523-9 PMID: 14946317
The purification of hypertensin I.
J Exp Med. 1954 Oct 1;100(4):363-70 PMID: 13201713
A method of assaying small amounts of hypertensin.
Lab Invest. 1953 Mar-Apr;2(2):109-14 PMID: 13045413
The purification of hog renin substrate.
J Biol Chem. 1954 Sep;210(1):281-6 PMID: 13201589
Corticotropins (ACTH). II. Amino acid composition of alpha-corticotropin.
J Biol Chem. 1955 Mar;213(1):187-96 PMID: 14353917
The isolation of hypertensin from the circulating blood of normal dogs with experimental renal hypertension by dialysis in an artificial kidney.
Circulation. 1951 Mar;3(3):384-9 PMID: 14812667
The amino acid composition of hypertensin II and its biochemical relationship to hypertensin I.
J Exp Med. 1956 Aug 1;104(2):183-91 PMID: 13345963
The existence of two forms of hypertensin.
J Exp Med. 1954 Mar;99(3):275-82 PMID: 13130799
The preparation and function of the hypertensin-converting enzyme.
J Exp Med. 1956 Mar 1;103(3):295-9 PMID: 13295487
Chromatography of amino acids on sulfonated polystyrene resins.
J Biol Chem. 1951 Oct;192(2):663-81 PMID: 14907661
The amino acid sequence of hypertensin. II.
J Exp Med. 1956 Aug 1;104(2):193-7 PMID: 13345964

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.106.3.439

Published

1957-09-01

Pages

439-53

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2136772

Subset

External Links

PubMed source DOI: 10.1084/jem.106.3.439 PMC Full Text View journal details

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The preparation, purification, and amino acid sequence of a polypeptide renin substrate.

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