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PMID: 1347144 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization and genetic mapping of a short, highly repeated, interspersed DNA sequence from rice (Oryza sativa L.).

Molecular & general genetics : MGG ·Vol. 231 ·No. 3 ·1992-02-00 ·Pages 353-9

Zhao X, Kochert G

Abstract

A short, highly repeated, interspersed DNA sequence from rice was characterized using a combination of techniques and genetically mapped to rice chromosomes by restriction fragment length polymorphism (RFLP) analysis. A consensus sequence (GGC)n, where n varies from 13-16, for the repeated sequence family was deduced from sequence analysis. Southern blot analysis, restriction mapping of repeat element-containing genomic clones, and DNA sequence analysis indicated that the repeated sequence is interspersed in the rice genome, and is heterogeneous and divergent. About 200,000 copies are present in the rice genome. Single copy sequences flanking the repeat element were used as RFLP markers to map individual repeat elements. Eleven such repeat elements were mapped to seven different chromosomes. The strategy for characterization of highly dispersed repeated DNA and its uses in genetic mapping, DNA fingerprinting, and evolutionary studies are discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Chromosome Mapping DNA/genetics Gene Library Molecular Sequence Data Open Reading Frames Oryza/genetics Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhao X
Department of Botany, University of Georgia, Athens 30602.
Kochert G
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00292702
Published
1992-02-00
Pages
353-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292702
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