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PMID: 1347946 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Extensive genetic diversity in the HLA class II region of Africans, with a focally predominant allele, DRB1*1304.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 6 ·1992-03-15 ·Pages 2277-81

Hill AV, Allsopp CE, Kwiatkowski D, Taylor TE, Yates SN, Anstey NM, Wirima JJ, Brewster DR, McMichael AJ, Molyneux ME

Abstract

Molecular HLA class II typing of greater than 1700 individuals from The Gambia in West Africa and Malawi in South-Central Africa revealed a striking diversity of HLA DRB-DQB haplotypes as defined by restriction fragment length polymorphism (RFLP); this diversity is twice as extensive as that found in northern Europeans. Despite this diversity, sequence and PCR/oligonucleotide analysis showed that the recently described variant DRB1*1304 is the commonest DRB1 allele in The Gambia. The sequence, geographical distribution, and RFLP association of this allele, together with homozygosity test results, suggest that DRB1*1304 may have arisen from DRB1*1102 and have reached its remarkably high frequency as a result of recent directional selection. The prevalence of this unusual allele has implications for trials of subunit vaccines in this area. The extensive and distinctive HLA class II region polymorphism in sub-Saharan Africans is consistent with evidence from other genetic loci implying an African origin of modern Homo sapiens.

MeSH Terms
Alleles Blacks/genetics DNA/blood,isolation & purification Gambia Gene Frequency Genes, MHC Class II Genetic Variation HLA-DR Antigens/genetics HLA-DRB1 Chains Haplotypes Histocompatibility Antigens Class II/genetics Homozygote Humans Leukocytes/immunology Polymorphism, Restriction Fragment Length Restriction Mapping
Chemicals
HLA-DR Antigens HLA-DRB1 Chains Histocompatibility Antigens Class II DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Hill A V
Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, United Kingdom.
Allsopp C E
Kwiatkowski D
Taylor T E
Yates S N
Anstey N M
Wirima J J
Brewster D R
McMichael A J
Molyneux M E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.6.2277
Published
1992-03-15
Pages
2277-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48640
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1073/pnas.89.6.2277 PMC Full Text View journal details
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