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PMID: 1356764 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A single amino acid substitution is sufficient to modify the mitogenic properties of the epidermal growth factor receptor to resemble that of gp185erbB-2.

The EMBO journal ·Vol. 11 ·No. 11 ·1992-11-00 ·Pages 3927-33

Di Fiore PP, Helin K, Kraus MH, Pierce JH, Artrip J, Segatto O, Bottaro DP

Abstract

The epidermal growth factor (EGF) receptor (EGFR) and the erbB-2 gene product, gp185erbB-2, exhibit distinct abilities to stimulate mitogenesis in different target cells. By using chimeric molecules between these two receptors, we have previously shown that their intracellular juxtamembrane regions are responsible for this specificity. Here we describe a genetically engineered EGFR mutant containing a threonine for arginine substitution at position 662 in the EGFR juxtamembrane domain, corresponding to threonine 694 in gp185erbB-2. This mutant, designated EGFRThr662, displayed affinity for EGF binding and catalytic properties that were indistinguishable from those of the wild type EGFR. However, EGFRThr662 behaved much as gp185erbB-2 in a number of bioassays which readily distinguish between the mitogenic effects of EGFR and gp185erbB-2. Moreover, significant differences were detected in the pattern of intracellular proteins phosphorylated on tyrosine in vivo by EGFR and EGFRThr662 in response to EGF. Thus, small differences in the primary sequence of two closely related receptors have dramatic effects on their ability to couple with mitogenic pathways.

Related Genes
MeSH Terms
3T3 Cells Amino Acid Sequence Animals Arginine Base Sequence Codon/genetics DNA Replication/drug effects Epidermal Growth Factor/metabolism,pharmacology ErbB Receptors/genetics,physiology Genetic Vectors Kinetics Mice Mitogens Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides Phosphorylation Protein-Tyrosine Kinases/genetics,metabolism Proto-Oncogene Proteins/genetics,physiology Proto-Oncogenes Receptor, ErbB-2 Repetitive Sequences, Nucleic Acid Signal Transduction Threonine Thymidine/metabolism Transfection
Chemicals
Codon Mitogens Oligodeoxyribonucleotides Proto-Oncogene Proteins Threonine Epidermal Growth Factor Arginine ErbB Receptors Protein-Tyrosine Kinases Receptor, ErbB-2 Thymidine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Di Fiore P P
Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Helin K
Kraus M H
Pierce J H
Artrip J
Segatto O
Bottaro D P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-11-00
Pages
3927-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556903
Subset
IM
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