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PMID: 1356992 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of tissue transglutaminase in Balb-C 3T3 fibroblasts: effects on cellular morphology and adhesion.

The Journal of cell biology ·Vol. 119 ·No. 2 ·1992-10-00 ·Pages 463-74

Gentile V, Thomazy V, Piacentini M, Fesus L, Davies PJ

Abstract

Tissue transglutaminase is a cytosolic enzyme whose primary function is to catalyze the covalent cross-linking of proteins. To investigate the functions of this enzyme in physiological systems, we have established lines of Balb-C 3T3 fibroblasts stably transfected with a constitutive tissue transglutaminase expression plasmid. Several cell lines expressing high levels of catalytically active tissue transglutaminase have been isolated and characterized. Transglutaminase-transfected cells showed morphologic features quite distinct from their nontransfected counterparts. Many of the cells showed an extended and very flattened morphology that reflected increased adhesion of the cells to the substratum. Other cells, particularly those showing the highest levels of intracellular transglutaminase expression, showed extensive membrane blebbing and cellular fragmentation. The results of these experiments suggest that the induction and activation of tissue transglutaminase may contribute both to changes in cellular morphology and adhesiveness.

MeSH Terms
3T3 Cells Animals Apoptosis/physiology Butyrates/pharmacology Butyric Acid Cell Adhesion/physiology Cell Division Enzyme Induction/drug effects Fibroblasts/physiology Fluorescent Antibody Technique Genetic Vectors Mice Mice, Inbred BALB C Morphogenesis/physiology Peptides/metabolism Phenotype Recombinant Proteins/biosynthesis Transfection Transglutaminases/biosynthesis,genetics,isolation & purification
Chemicals
Butyrates Peptides Recombinant Proteins Butyric Acid Transglutaminases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gentile V
Department of Pharmacology, Medical School, University of Texas Health Science Center, Houston.
Thomazy V
Piacentini M
Fesus L
Davies P J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.2.463
Published
1992-10-00
Pages
463-74
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289646
Subset
IM
Grants
NIDDK NIH HHS · DK1-27078 · United States
External Links
PubMed source DOI: 10.1083/jcb.119.2.463 PMC Full Text View journal details
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