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PMID: 1357539 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Activation of the Rhizobium leguminosarum glnII gene by NtrC is dependent on upstream DNA sequences.

Molecular & general genetics : MGG ·Vol. 234 ·No. 3 ·1992-09-00 ·Pages 337-45

Patriarca EJ, Chiurazzi M, Manco G, Riccio A, Lamberti A, De Paolis A, Rossi M, Defez R, Iaccarino M

Abstract

The cloning and sequence determination is reported of the DNA region of Rhizobium leguminosarum coding for glutamine synthetase II (GSII). An open reading frame (ORF) encoding 326 amino acids was defined as the glnII gene on the basis of its similarity to other glnII genes and the ability of a DNA fragment carrying this ORF to complement the glutamine auxotrophy of a Klebsiella pneumoniae glnA mutant. We find that the glnII gene in R. leguminosarum is transcribed as a monocistronic unit from a single promoter, which shows structural features characteristic of rpoN (ntrA)-dependent promoters. In K. pneumoniae, such promoters require the ntrC and rpoN (ntrA) gene products for transcription. The intracellular level of glnII mRNA changes when R. leguminosarum is grown on different nitrogen sources, as expected for regulation by the nitrogen regulatory system. Promoter deletion analysis has shown that an extensive upstream DNA sequence (316 bp) is essential for in vivo activation of the glnII promoter in different biovars of R. leguminosarum. This DNA region requires a wild-type ntrC gene for activity and includes two conserved putative NtrC-binding site sequences. The results conclusively show that transcription from the R. leguminosarum glnII promoter is fully dependent on positive control by NtrC protein and on an upstream activator sequence (UAS).

Related Genes
glnII
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Consensus Sequence DNA, Bacterial/genetics DNA-Binding Proteins/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Glutamate-Ammonia Ligase/genetics Klebsiella pneumoniae/genetics Molecular Sequence Data PII Nitrogen Regulatory Proteins Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Restriction Mapping Rhizobium leguminosarum/genetics Sequence Alignment Trans-Activators Transcription Factors/genetics Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins PII Nitrogen Regulatory Proteins Trans-Activators Transcription Factors Glutamate-Ammonia Ligase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Patriarca E J
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Chiurazzi M
Manco G
Riccio A
Lamberti A
De Paolis A
Rossi M
Defez R
Iaccarino M
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00538692
Published
1992-09-00
Pages
337-45
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X67296
External Links
PubMed source DOI: 10.1007/BF00538692
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