Characterization of a factor formed in the course of adenovirus infection of tissue cultures causing detachment of cells from glass.

The Journal of experimental medicine ·Vol. 108 ·No. 5 ·1958-11-01 ·Pages 713-29

ROWE WP, HARTLEY JW, ROIZMAN B, LEVY HB

Abstract

Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and responsible for cell detachment is trypsin-sensitive and non-dialyzable; it is smaller and more resistant to the effect of heat or ultraviolet light than the infectious virus particle. Cell detachment activity was found to be temperature-dependent, and the cell-detaching titer of infectious tissue culture fluids was not affected by repeated exposure to HeLa cells. Inhibition of cell detachment by human or rabbit sera was observed only when other antibodies to adenovirus antigens were also present, but the antibody inhibiting cell detachment could not be correlated quantitatively with complement-fixing or homologous neutralizing antibody.

Keywords

ADENOVIRUS

MeSH Terms

Adenoviridae Adenoviridae Infections Animals Antigens, Viral Humans Rabbits

Chemicals

Antigens, Viral

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

ROWE W P

HARTLEY J W

ROIZMAN B

LEVY H B

References (13)

13 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.108.5.713

Published

1958-11-01

Pages

713-29

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2136912

Subset

External Links

PubMed source DOI: 10.1084/jem.108.5.713 PMC Full Text View journal details

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Characterization of a factor formed in the course of adenovirus infection of tissue cultures causing detachment of cells from glass.

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