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PMID: 1361173 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA.

The EMBO journal ·Vol. 11 ·No. 13 ·1992-12-00 ·Pages 5111-20

Waseem NH, Labib K, Nurse P, Lane DP

Abstract

Five monoclonal antibodies raised against rat PCNA cross-reacted with a similar protein in the fission yeast Schizosaccharomyces pombe. One of these was used to screen an S.pombe cDNA expression library. An incomplete cDNA was isolated and used to screen a genomic library, identifying a single gene, designated pcn1+ (proliferating cell nuclear antigen). The gene encodes a protein of 260 amino acids, with a deduced sequence 52% identical to human and rat PCNAs, which are 98.5% identical to each other. The budding yeast PCNA homologue POL30 is only 35% identical to the human and rat proteins. Pcn1 has a region near the C-terminus of particularly high homology to higher eukaryotic PCNA proteins. pcn1+ is essential for viability and delta pcn1 cells undergo aberrant DNA replication before cell cycle arrest. Overproduction of the protein leads to cell cycle delay in G2. Disruption of pcn1+ is complemented by the human PCNA gene, demonstrating that these genes are functional homologues.

Related Genes
PCNA POL30
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA Replication/genetics DNA, Fungal Fungal Proteins/genetics Genes, Fungal Genetic Complementation Test Humans Molecular Sequence Data Nuclear Proteins/genetics Plasmids Proliferating Cell Nuclear Antigen RNA-Binding Proteins Rats Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Spores, Fungal
Chemicals
DNA, Fungal Fungal Proteins Nuclear Proteins PCN1 protein, S pombe Proliferating Cell Nuclear Antigen RNA-Binding Proteins Schizosaccharomyces pombe Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Waseem N H
CRC Cell Transformation Research Group, University of Dundee, UK.
Labib K
Nurse P
Lane D P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-12-00
Pages
5111-20
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556989
Subset
IM
Databases
GENBANK
D13199, D13200, D13201, D13202, D13203, M94783, M94784, M94785, X54857, X68109
External Links
PubMed source PMC Full Text View journal details
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