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PMID: 13630927 Published · ppublish English Journal Article

A cytochemical study on the pancreas of the guinea pig. IV. Chemical and metabolic investigation of the ribonucleoprotein particles.

The Journal of biophysical and biochemical cytology ·Vol. 5 ·No. 1 ·1959-01-25 ·Pages 1-10

SIEKEVITZ P, PALADE GE

Abstract

Five ribonucleoprotein (RNP) fractions were isolated from the postmitochondrial supernatant of the pancreas of the guinea pig. Two were obtained from the microsomes which, by deoxycholate (DOC) treatment, were subdivided into a DOC-soluble and a DOC-insoluble fraction. The latter was taken to represent attached RNP particles. Two other fractions obtained from the microsomal supernatant supposedly represent free RNP particles existing as such in the cytoplasm, while a third fraction resisted sedimentation for 20 hours at 105,000 g and is considered to be a soluble nucleoprotein. These fractions exhibited different RNA/protein ratios and also different RNA turnover patterns, as determined after in vivo labelling with adenine-8-C(14). However, little discernible differences could be detected in the nucleotide composition of the RNA moieties of these RNP fractions. Amino acid-"activating" enzymes were found to occur in the fraction containing the soluble nucleoproteins. The discussion focuses on the relationships between these fractions and protein synthesis in the pancreas, using data given in this and a previous paper, and data contained in the literature.

Keywords
NUCLEOPROTEINS/metabolism PANCREAS/metabolism
MeSH Terms
Adenine Amino Acids Cytoplasm Guinea Pigs Microsomes Nucleoproteins/metabolism Pancreas/metabolism Protein Biosynthesis RNA Ribonucleoproteins
Chemicals
Amino Acids Nucleoproteins Ribonucleoproteins RNA Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
SIEKEVITZ P
PALADE G E
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49 references, click to expand
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Article Info
Journal
The Journal of biophysical and biochemical cytology
Abbr.
J Biophys Biochem Cytol
ISSN
0095-9901
DOI
10.1083/jcb.5.1.1
Published
1959-01-25
Pages
1-10
Language
English
Region
United States
NLM ID
17840020R
PMCID
PMC2224619
Subset
OM
External Links
PubMed source DOI: 10.1083/jcb.5.1.1 PMC Full Text
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