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PMID: 1367777 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Production of heterologous proteins in Bacillus subtilis: the effect of the joint between signal sequence and mature protein on yield.

Applied microbiology and biotechnology ·Vol. 36 ·No. 1 ·1991-10-00 ·Pages 61-4

Hemilä H, Sibakov M

Abstract

We have previously made a set of DNA constructs by fusing the mature part of Bacillus licheniformis alpha-amylase with the signal sequence of B. amyloliquefaciens alpha-amylase at various distances from the signal sequence cleavage site. We observed that the level of alpha-amylase production in B. subtilis depended strongly on the distance of the junction from the signal sequence cleavage site, with quite a sharp optimum distance. To test whether the effect is limited to the pair of alpha-amylase signal sequence and mature protein, we analysed the protein production in a set of constructs in which an Escherichia coli beta-lactamase was similarly joined at different distances from the alpha-amylase signal sequence. Also in this case the distance seemed to be an important factor in affecting the level of production in B. subtilis. The observed effect might depend on the modulation of pre-protein folding, which in turn could affect the secretion level.

MeSH Terms
Amino Acid Sequence Bacillus/genetics Bacillus subtilis/genetics,metabolism Bacterial Proteins/biosynthesis,genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Molecular Sequence Data Protein Sorting Signals/biosynthesis,genetics Recombinant Fusion Proteins/biosynthesis alpha-Amylases/biosynthesis,genetics beta-Lactamases/biosynthesis,genetics
Chemicals
Bacterial Proteins Protein Sorting Signals Recombinant Fusion Proteins alpha-Amylases beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hemilä H
Institute of Biotechnology, Helsinki, Finland.
Sibakov M
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22 references, click to expand
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Article Info
Journal
Applied microbiology and biotechnology
Abbr.
Appl Microbiol Biotechnol
ISSN
0175-7598
DOI
10.1007/BF00164699
Published
1991-10-00
Pages
61-4
Language
English
Region
Germany
NLM ID
8406612
External Links
PubMed source DOI: 10.1007/BF00164699 View journal details
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