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PMID: 13679984 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

First RFLP linkage map of red clover ( Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 108 ·No. 1 ·2003-12-00 ·Pages 105-12

Isobe S, Klimenko I, Ivashuta S, Gau M, Kozlov NN

Abstract

We constructed a genetic linkage map of red clover ( Trifolium pratense L., 2n=2 x=14) using RFLP markers from cDNA probes of a backcrossed mapping population, and investigated the transferability of the markers to other red clover germplasm. The map contains 157 RFLP markers and one morphological marker on seven linkage groups. The total map distance was 535.7 cM and the average distance between two markers was 3.4 cM. All of the cDNA probes of the map were hybridized to the fragments of genomic DNA from 12 plants derived from three varieties, and 87% of the cDNA probes detected polymorphic bands that corresponded to those of mapping parents. This result indicated that RFLP markers on the present map were transferable to the genome analysis of other red clover germplasm. This is the first report to construct a linkage map of Trifolium species; it should provide fundamental and useful genetic information relevant to the breeding of red clover and genus Trifolium.

MeSH Terms

Chromosome Mapping Chromosome Segregation Crosses, Genetic DNA, Complementary DNA, Plant/genetics Genes, Plant Genetic Linkage Genetic Markers Inbreeding Polymorphism, Genetic Polymorphism, Restriction Fragment Length Quantitative Trait Loci Trifolium/genetics

Chemicals

DNA, Complementary DNA, Plant Genetic Markers

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Isobe S

National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo, 062-8555, Hokkaido, Japan. [email protected]

Klimenko I

Ivashuta S

Gau M

Kozlov N N

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Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/s00122-003-1412-z

[email protected]

Published

2003-12-00

Epub

2003-00-16

Pages

105-12

Language

English

Region

Germany

NLM ID

0145600

Subset

External Links

PubMed source DOI: 10.1007/s00122-003-1412-z View journal details

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First RFLP linkage map of red clover ( Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm.

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