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PMID: 1369331 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA recovery and direct detection of Tn5 sequences from soil.

Letters in applied microbiology ·Vol. 13 ·No. 1 ·1991-07-00 ·Pages 21-4

Selenska S, Klingmüller W

Abstract

Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.

MeSH Terms

DNA/analysis DNA Transposable Elements/genetics Deoxyribonuclease EcoRI Enterobacter/genetics Soil/analysis Soil Microbiology

Chemicals

DNA Transposable Elements Soil DNA Deoxyribonuclease EcoRI

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Selenska S

Department of Genetics, University of Bayreuth, Germany.

Klingmüller W

Article Info

Journal

Letters in applied microbiology

Abbr.

Lett Appl Microbiol

ISSN

0266-8254

DOI

10.1111/j.1472-765x.1991.tb00559.x

Published

1991-07-00

Pages

21-4

Language

English

Region

England

NLM ID

8510094

External Links

PubMed source DOI: 10.1111/j.1472-765x.1991.tb00559.x View journal details

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DNA recovery and direct detection of Tn5 sequences from soil.

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