The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes.

Letters in applied microbiology ·Vol. 13 ·No. 3 ·1991-09-00 ·Pages 171-4

Embley TM

Abstract

The linear polymerase chain reaction was used to sequence amplified RNA genes from strains of Bacillus, Thermus and Legionella. The technique described is simple and reproducible and it works well with double standard product which has been PEG precipitated directly from PCR reactions.

MeSH Terms

Bacillus/genetics Base Sequence Gene Amplification Legionella/genetics Molecular Sequence Data Polymerase Chain Reaction RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry Templates, Genetic Thermus/genetics

Chemicals

RNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Embley T M

Department of Zoology, Natural History Museum, London, UK.

Article Info

Journal

Letters in applied microbiology

Abbr.

Lett Appl Microbiol

ISSN

0266-8254

DOI

10.1111/j.1472-765x.1991.tb00600.x

Published

1991-09-00

Pages

171-4

Language

English

Region

England

NLM ID

8510094

External Links

PubMed source DOI: 10.1111/j.1472-765x.1991.tb00600.x View journal details

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