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PMID: 13715795 Published · ppublish English Journal Article

The fine structure of the nuclear material of a blue-green alga, Anabaena cylindrica Lemm.

The Journal of biophysical and biochemical cytology ·Vol. 8 ·1960-12-00 ·Pages 813-23

HOPWOOD DA, GLAUERT AM

Abstract

The chromatinic material of the blue-green alga Anabaena cylindrica has complex configurations in the central regions of the cells. The distribution of the chromatin within the cells varies in different filaments, probably in response to variations in the disposition of other cellular components. In electron micrographs of thin sections of organisms fixed by the method of Kellenberger, Ryter, and Séchaud (1958) the centroplasm contains fibrillar and possibly granular components which can be identified as the nuclear material by comparison with stained preparations viewed in the light microscope. The fibrils in the nuclear regions have diameters in the range of 5 to 7 mmicro and are embedded in a matrix of lower density. The nuclear regions are not greatly different from the cytoplasm in their electron density. Reducing the calcium content of the fixative results in coagulation of the fibrils to form coarser structures. The significance of the observations is discussed in relation to observations on the fine structure of other classes of algae and of bacteria.

Keywords
ALGAE/anatomy and histology CELL NUCLEUS
MeSH Terms
Anabaena cylindrica Bacteria Cell Nucleolus Cell Nucleus Chromatin Cytoplasm Cytoskeleton Diploidy Eukaryota/anatomy & histology Fixatives Histological Techniques Microscopy Plants
Chemicals
Chromatin Fixatives
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
HOPWOOD D A
GLAUERT A M
References (13)
13 references, click to expand
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Article Info
Journal
The Journal of biophysical and biochemical cytology
Abbr.
J Biophys Biochem Cytol
ISSN
0095-9901
Published
1960-12-00
Pages
813-23
Language
English
Region
United States
NLM ID
17840020R
PMCID
PMC2224952
Subset
OM
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