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PMID: 1372620 Published · ppublish English Comparative Study Journal Article

Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes.

Journal of clinical microbiology ·Vol. 30 ·No. 3 ·1992-03-00 ·Pages 628-32

Marconi RT, Lubke L, Hauglum W, Garon CF

Abstract

Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cultures was used in slot blot analysis. Radiolabeled oligonucleotides designed to hybridize to specific 16S rRNA targets were used as probes. These probes allowed for both species-specific identification and genomic group typing of B. burgdorferi.

MeSH Terms
Bacterial Typing Techniques Base Sequence Borrelia burgdorferi Group/classification,genetics,isolation & purification DNA Probes Evaluation Studies as Topic Humans Lyme Disease/microbiology Molecular Sequence Data Oligodeoxyribonucleotides/genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Species Specificity
Chemicals
DNA Probes Oligodeoxyribonucleotides RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marconi R T
Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.
Lubke L
Hauglum W
Garon C F
References (30)
30 references, click to expand
  1. Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi.
    J Bacteriol. 1992 Jan;174(1):241-4 PMID: 1370282
  2. Antigenic variation of Borrelia hermsii.
    J Exp Med. 1982 Nov 1;156(5):1297-311 PMID: 7130900
  3. Detection of Borrelia burgdorferi using the polymerase chain reaction.
    J Clin Microbiol. 1990 Jun;28(6):1089-93 PMID: 2199482
  4. The 83-kilodalton antigen of Borrelia burgdorferi which stimulates immunoglobulin M (IgM) and IgG responses in infected hosts is expressed by a chromosomal gene.
    J Clin Microbiol. 1990 Jul;28(7):1673-5 PMID: 2380391
  5. Detection of Borrelia burgdorferi DNA in museum specimens of Ixodes dammini ticks.
    Science. 1990 Sep 21;249(4975):1420-3 PMID: 2402635
  6. Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species.
    J Gen Microbiol. 1987 Jul;133(7):1969-74 PMID: 2444670
  7. Lyme disease.
    N Engl J Med. 1989 Aug 31;321(9):586-96 PMID: 2668764
  8. Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species.
    J Clin Microbiol. 1988 Dec;26(12):2484-8 PMID: 3230127
  9. Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation.
    Infect Immun. 1988 Aug;56(8):1831-6 PMID: 3397175
  10. Biology of Borrelia species.
    Microbiol Rev. 1986 Dec;50(4):381-400 PMID: 3540570
  11. Antigenic changes of Borrelia burgdorferi as a result of in vitro cultivation.
    J Infect Dis. 1987 Nov;156(5):852-3 PMID: 3655406
  12. Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):278-82 PMID: 6572002
  13. Identification of a third genomic group of Borrelia burgdorferi through signature nucleotide analysis and 16S rRNA sequence determination.
    J Gen Microbiol. 1992 Mar;138(3):533-6 PMID: 1593264
  14. Detection and identification of Treponema hyodysenteriae by using oligodeoxynucleotide probes complementary to 16S rRNA.
    J Clin Microbiol. 1990 Dec;28(12):2717-21 PMID: 1704013
  15. Comparison of in vitro culture and polymerase chain reaction for detection of Borrelia burgdorferi in tissue from experimentally infected animals.
    J Clin Microbiol. 1991 Apr;29(4):731-7 PMID: 1890174
  16. Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi.
    J Clin Microbiol. 1991 Mar;29(3):524-32 PMID: 2037670
  17. Characterization of an rRNA gene-specific cDNA probe: applications in bacterial identification.
    J Gen Microbiol. 1990 Aug;136(8):1639-43 PMID: 2262795
  18. Reactivity of human Lyme borreliosis sera with a 39-kilodalton antigen specific to Borrelia burgdorferi.
    J Clin Microbiol. 1990 Jun;28(6):1329-37 PMID: 2380361
  19. Two genomic species in Borrelia burgdorferi.
    Res Microbiol. 1990 May;141(4):465-75 PMID: 2399368
  20. Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates.
    J Infect Dis. 1985 Sep;152(3):478-84 PMID: 2411827
  21. Antigenic variability of Borrelia burgdorferi.
    Ann N Y Acad Sci. 1988;539:126-43 PMID: 2461135
  22. Antigenic heterogeneity of European Borrelia burgdorferi strains isolated from patients and ticks.
    Lancet. 1985 May 11;1(8437):1099 PMID: 2582217
  23. DNA probes for the identification of Haemophilus ducreyi.
    J Clin Microbiol. 1989 Jul;27(7):1441-5 PMID: 2788660
  24. New infectious spirochete isolated from short-tailed shrews and white-footed mice.
    J Clin Microbiol. 1987 Aug;25(8):1490-4 PMID: 3305565
  25. Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California.
    J Clin Microbiol. 1987 Dec;25(12):2296-301 PMID: 3323225
  26. A Borrelia-specific monoclonal antibody binds to a flagellar epitope.
    Infect Immun. 1986 May;52(2):549-54 PMID: 3516878
  27. The enlarging spectrum of tick-borne spirochetoses: R. R. Parker Memorial Address.
    Rev Infect Dis. 1986 Nov-Dec;8(6):932-40 PMID: 3541127
  28. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  29. Isolation and cultivation of Lyme disease spirochetes.
    Yale J Biol Med. 1984 Jul-Aug;57(4):521-5 PMID: 6393604
  30. Molecular detection of persistent Borrelia burgdorferi in the urine of patients with active Lyme disease.
    Infect Immun. 1991 Jan;59(1):269-78 PMID: 1987041
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.3.628-632.1992
Published
1992-03-00
Pages
628-32
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265123
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.3.628-632.1992 PMC Full Text View journal details
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