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PMID: 1372803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phosphorylation of elongation factor 2 during Ca(2+)-mediated secretion from rat parotid acini.

The Biochemical journal ·Vol. 282 ( Pt 3) ·1992-03-15 ·Pages 877-82

Hincke MT, Nairn AC

Abstract

In this paper we report the rapid phosphorylation of a cytosolic 100 kDa protein during stimulation of secretion from dispersed aggregates of parotid acinar cells with Ca(2+)-mobilizing secretagogues (carbachol, Substance P, ATP and the Ca2+ ionophore A23187). Phosphorylation was inhibited by removal of extracellular Ca2+ but was not observed during stimulation with phorbol esters, suggesting that this protein is not a substrate for protein kinase C. Two-dimensional PAGE and immunoprecipitation with a specific antiserum indicated that this protein is elongation factor 2, whose Ca2+ calmodulin-dependent phosphorylation has been shown to inhibit protein synthesis [Nairn & Palfrey (1987) J. Biol. Chem. 262, 17299-17303]. These results suggest that phosphorylation of elongation factor 2 is the molecular mechanism for the inhibition of protein synthesis which has been previously observed in rat parotid cells during stimulation with Ca(2+)-mobilizing secretagogues.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Autoradiography Calcimycin/pharmacology Calcium/metabolism,physiology Carbachol/pharmacology Kinetics Male Parotid Gland/drug effects,metabolism Peptide Elongation Factor 2 Peptide Elongation Factors/metabolism Phosphorylation Rats Rats, Inbred Strains Stimulation, Chemical Substance P/pharmacology
Chemicals
Peptide Elongation Factor 2 Peptide Elongation Factors Substance P Calcimycin Adenosine Triphosphate Carbachol Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hincke M T
Department of Anatomy, University of Ottawa, Ontario, Canada.
Nairn A C
References (43)
43 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2820877
Published
1992-03-15
Pages
877-82
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1130869
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2820877 PMC Full Text View journal details
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