Home LiteratureArticle Details
PMID: 1374836 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of a CpA methylase in an insect system: characterization and substrate specificity.

Molecular and cellular biochemistry ·Vol. 110 ·No. 2 ·1992-03-25 ·Pages 103-11

Devajyothi C, Brahmachari V

Abstract

A cytosine-specific DNA methyltransferase (EC 2.1.1.37) has been purified to near homogeneity from a mealybug (Planococcus lilacinus). The enzyme can methylate cytosine residues in CpG sequences as well as CpA sequences. The apparent molecular weight of the enzyme was estimated as 135,000 daltons by FPLC. The enzyme exhibits a processive mode of action and a salt dependence similar to mammalian methylases. Mealybug methylase exhibits a preference for denatured DNA substrates.

MeSH Terms
Animals Coleoptera/enzymology DNA-Cytosine Methylases/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Female Kinetics Male Methylation Potassium Chloride/pharmacology RNA/metabolism Substrate Specificity
Chemicals
RNA Potassium Chloride DNA-Cytosine Methylases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Devajyothi C
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Brahmachari V
References (31)
31 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Absence of cytosine methylation at C-C-G-G and G-C-G-C sites in the rDNA coding regions and intervening sequences of Drosophila and the rDNA of other insects.
    Nucleic Acids Res. 1979 Jul 11;6(9):2987-95 PMID: 493131
  3. DNA modification mechanisms and gene activity during development.
    Science. 1975 Jan 24;187(4173):226-32 PMID: 1111098
  4. Eukaryotic DNA methylase--properties and action on native DNA and chromatin.
    Curr Top Microbiol Immunol. 1984;108:142-56 PMID: 6325096
  5. Isolation and characterization of DNA cytosine 5-methyltransferase from human placenta.
    Biochim Biophys Acta. 1983 Aug 2;740(3):323-30 PMID: 6409149
  6. DNA-methylase from regenerating rat liver: purification and characterisation.
    Nucleic Acids Res. 1978 Jun;5(6):2153-67 PMID: 673848
  7. Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II.
    Science. 1979 Mar 9;203(4384):1019-21 PMID: 424726
  8. Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA.
    FEBS Lett. 1983 Jul 25;158(2):353-8 PMID: 6409666
  9. Mouse ascites DNA methylase: characterisation of size, proteolytic breakdown and nucleotide recognition.
    Biochim Biophys Acta. 1986 Oct 16;868(1):9-16 PMID: 3756172
  10. DNA methylase from HeLa cell nuclei.
    Nucleic Acids Res. 1975 Oct;2(10):1669-84 PMID: 1187340
  11. Mouse DNA methylase: methylation of native DNA.
    Biochim Biophys Acta. 1979 Feb 27;561(2):345-57 PMID: 427161
  12. X inactivation, differentiation, and DNA methylation.
    Cytogenet Cell Genet. 1975;14(1):9-25 PMID: 1093816
  13. Preferential binding of DNA methyltransferase and increased de novo methylation of deoxyinosine containing DNA.
    FEBS Lett. 1986 Oct 20;207(1):75-8 PMID: 3770195
  14. DNA methylation and gene activity.
    Annu Rev Biochem. 1983;52:93-124 PMID: 6311083
  15. DNA sequences showing a delay in cytosine methylation after replication. Time course of methylation in synchronized mammalian cell populations and relationship to DNAase I sensitive domains.
    Biochim Biophys Acta. 1983 Oct 13;741(1):38-46 PMID: 6225463
  16. Parental legacy determines methylation and expression of an autosomal transgene: a molecular mechanism for parental imprinting.
    Cell. 1987 Aug 28;50(5):719-27 PMID: 3040259
  17. A methylation mosaic model for mammalian genome imprinting.
    Prog Nucleic Acid Res Mol Biol. 1989;36:145-57 PMID: 2660189
  18. Chromatography of nucleic acids on hydroxyapatite.
    Nature. 1965 May 22;206(4986):779-83 PMID: 5840127
  19. A sensitive immunochemical method for detecting 5mC in DNA fragments.
    FEBS Lett. 1982 Dec 27;150(2):469-72 PMID: 6819163
  20. Immunologic studies on nucleic acids and their components. II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification.
    Biochim Biophys Acta. 1974 Apr 27;349(1):84-95 PMID: 11400441
  21. Retrovirus genomes methylated by mammalian but not bacterial methylase are non-infectious.
    Nature. 1983 Jul 21-27;304(5923):275-7 PMID: 6306480
  22. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  23. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  24. DNA methylation. Inhibition of de novo and maintenance methylation in vitro by RNA and synthetic polynucleotides.
    J Biol Chem. 1984 Oct 25;259(20):12437-43 PMID: 6208188
  25. Mechanism of action of rat liver DNA methylase. II. Interaction with single-stranded methyl-acceptor DNA.
    J Mol Biol. 1971 Oct 28;61(2):343-56 PMID: 5167086
  26. DNA methylation and its possible biological roles.
    Prog Nucleic Acid Res Mol Biol. 1981;25:33-52 PMID: 6261297
  27. Mechanism of action of rat liver DNA methylase. I. Interaction with double-stranded methyl-acceptor DNA.
    J Mol Biol. 1971 May 14;57(3):475-89 PMID: 4931679
  28. DNA methylase: purification from ascites cells and the effect of various DNA substrates on its activity.
    Nucleic Acids Res. 1976 Mar;3(3):677-95 PMID: 131936
  29. Two DNA methyltransferases from murine erythroleukemia cells: purification, sequence specificity, and mode of interaction with DNA.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5559-63 PMID: 6577443
  30. Selective silencing of eukaryotic DNA.
    Science. 1975 Aug 8;189(4201):426-33 PMID: 1098145
  31. Mammalian DNA methyltransferases prefer poly(dI-dC) as substrate.
    J Biol Chem. 1986 Jun 15;261(17):7600-2 PMID: 3711099
Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF02454187
Published
1992-03-25
Pages
103-11
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02454187 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2