Mast cell degranulation and histamine release observed in a new in vitro system.

The Journal of experimental medicine ·Vol. 112 ·1960-10-01 ·Pages 571-80

LAGUNOFF D, BENDITT EP

Abstract

A method is described by which mast cells harvested from the rat peritoneal cavity can be deposited on the surface of a Millipore filter without gross injury, permitting observation of morphological and chemical changes induced by a variety of agents. These isolated peritoneal mast cells respond to 48/80 and to polymyxin B but not to dextran or ovomucoid with degranulation and histamine release. Thus four agents which in vivo appear to have similar activities have been found by means of in vitro analysis to operate by at least two different mechanisms.

Keywords

HISTAMINE MAST CELLS/physiology

MeSH Terms

Animals Antigen Presentation Cell Degranulation Histamine Histamine Release In Vitro Techniques Mast Cells/physiology Peritoneal Cavity Rats

Chemicals

Histamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

LAGUNOFF D

BENDITT E P

References (16)

16 references, click to expand

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Application of millipore filtration technique to cytologic material.
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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.112.4.571

Published

1960-10-01

Pages

571-80

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2137246

Subset

External Links

PubMed source DOI: 10.1084/jem.112.4.571 PMC Full Text View journal details

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Mast cell degranulation and histamine release observed in a new in vitro system.

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