Glycogen synthesis from uridine diphosphate glucose. The distribution of the enzyme in liver cell fractions.
The distribution in liver cell fractions of UDPG-glycogen transferase has been studied. In fasting animals which have been refed 6 hours before sacrifice, the distribution of the enzyme in the various cell fractions can be correlated with the glycogen content of each fraction. A purified glycogen fraction has been prepared by differential centrifugation in sucrose gradients. This glycogen fraction contains vesicular structures which resemble those seen in association with glycogen deposits in the intact liver cell. In addition, the glycogen pellet contains UDPG-glycogen transferase in high specific activity. Subfractionation of the glycogen pellet separates the majority of vesicular elements from the bulk of transferase activity and glycogen. The evidence presented suggests that the presence of UPDG-glycogen transferase in the glycogen pellet is to be attributed to its binding to glycogen rather than to its association with the structural elements found in the glycogen fraction.
28 references, click to expand
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[Role of the ergastoplasm in the synthesis of glycogen during the course of formation of the "paraboloid" of visual cells].C R Hebd Seances Acad Sci. 1960 Jan 18;250:600-2 PMID: 13807601
-
GLYCOGEN SYNTHESIS FROM GLUCOSE, GLUCOSE-6-PHOSPHATE, AND URIDINE DIPHOSPHATE GLUCOSE IN MUSCLE PREPARATIONS.Proc Natl Acad Sci U S A. 1959 Jan;45(1):6-12 PMID: 16590357
-
A FUNCTIONAL DISORDER OF MUSCLE ASSOCIATED WITH THE ABSENCE OF PHOSPHORYLASE.Proc Natl Acad Sci U S A. 1959 Jun;45(6):791-7 PMID: 16590445
-
Preparation and properties of uridinediphosphoglucoseglycogen transferase from rabbit muscle.Biochim Biophys Acta. 1959 Jun;33(2):556-9 PMID: 13670931
-
Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium.J Biophys Biochem Cytol. 1958 Nov 25;4(6):727-30 PMID: 13610936
-
The binding of glycogen and phosphorylase.J Biol Chem. 1958 Dec;233(6):1251-6 PMID: 13610823
-
Electron-microscopic observations on developing chick embryo liver. The Golgi complex and its possible role in the formation of glycogen.J Ultrastruct Res. 1960 Nov;4:149-65 PMID: 13751590
-
Cytochemical studies of mitochondria. I. The separation and identification of a membrane fraction from isolated mitochondria.J Biophys Biochem Cytol. 1956 Nov 25;2(6):639-52 PMID: 13398435
-
Cell morphology and the organization of enzymatic systems in cytoplasm.Proc R Soc Lond B Biol Sci. 1954 Mar 25;142(907):177-86 PMID: 13167066
-
An improved micromethod of phosphate determination.J Biol Chem. 1952 Sep;198(1):221-7 PMID: 12999735
-
The estimation of glycogen with the anthrone reagent.Arch Biochem. 1950 Jan;25(1):191-200 PMID: 15401229
-
A uridine coenzyme-linked pathway of glycogen synthesis in muscle.Biochim Biophys Acta. 1958 Nov;30(2):449 PMID: 13607478
-
Electron-microscopic study of glycogen in chick embryo liver.J Ultrastruct Res. 1960 Nov;4:191-212 PMID: 13751591
-
Glycogen metabolism.Physiol Rev. 1960 Jul;40:505-37 PMID: 13834511
-
Studies of liver glucose 6-phosphatase. III. Solubilization and properties of the enzyme from normal and diabetic rats.J Biol Chem. 1959 Aug;234(8):1937-41 PMID: 13672991
-
The metabolism of oligosaccharides.J Biol Chem. 1960 Nov;235:3058-60 PMID: 13730674
-
The relationship of epinephrine and glucagon to liver phosphorylase. IV. Effect of epinephrine and glucagon on the reactivation of phosphorylase in liver homogenates.J Biol Chem. 1957 Jan;224(1):463-75 PMID: 13398422
-
Levels of activity of the enzymes of the glycogen cycle in rat tissues.Arch Biochem Biophys. 1960 Feb;86:270-3 PMID: 13842295
-
Synthesis of glycogen from uridine diphosphate glucose in liver.J Biol Chem. 1960 Apr;235:919-23 PMID: 14415527
-
[The hexose-phosphatase system. III. Intracellular localization of enzymes by fractional centrifugation].Bull Soc Chim Biol (Paris). 1951;33(1-2):21-41 PMID: 14935564
-
Isolation of cytoplasmic pituitary granules with gonadotropic activity.J Biophys Biochem Cytol. 1960 Apr;7:209-18 PMID: 14400127
-
A study of fixation for electron microscopy.J Exp Med. 1952 Mar;95(3):285-98 PMID: 14927794
-
Biosynthesis of glycogen from uridine diphosphate glucose.Arch Biochem Biophys. 1959 Apr;81(2):508-20 PMID: 13638016
-
GLYCOGEN SYNTHESIS IN MUSCLE LACKING PHOSPHORYLASE.Proc Natl Acad Sci U S A. 1959 Aug;45(8):1236-40 PMID: 16590499
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Identification of glycogen in electron micrographs of thin tissue sections.J Biophys Biochem Cytol. 1960 Dec;8:575-89 PMID: 13741016
-
An electron microscope study of the early effects of 3'-Me-DAB on rat liver cells.Cancer Res. 1959 Nov;19:997-1009 PMID: 14434277
-
Liver microsomes; an integrated morphological and biochemical study.J Biophys Biochem Cytol. 1956 Mar 25;2(2):171-200 PMID: 13319380
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