The direct isolation of the mitotic apparatus.

The Journal of biophysical and biochemical cytology ·Vol. 10 ·1961-08-00 ·Pages 467-74

MAZIA D, MITCHISON JM, MEDINA H, HARRIS P

Abstract

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

Keywords

CELL DIVISION

MeSH Terms

Animals Cell Division Ovum Sea Urchins Spindle Apparatus Sucrose

Chemicals

Sucrose

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

MAZIA D

MITCHISON J M

MEDINA H

HARRIS P

References (11)

11 references, click to expand

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Proc Natl Acad Sci U S A. 1952 Sep;38(9):826-38 PMID: 16589185
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On the physical properties of the mitotic spindle.
Ann N Y Acad Sci. 1960 Oct 7;90:529-30 PMID: 13717779
Studies on the mitotic apparatus of the sea urchin by means of antigen-antibody reactions in agar.
J Biophys Biochem Cytol. 1959 Dec;6:447-55 PMID: 13843993
[Electron microscopic study of the ultrastructure of centrioles in vertebra].
Z Zellforsch Mikrosk Anat. 1956;45(3):378-98 PMID: 13402087
An interference microscopical and cytochemical analysis of local mass changes in the mitotic apparatus during mitosis.
Exp Cell Res. 1959 Mar;16(3):575-83 PMID: 13653025
SH compounds in mitosis. II. The effect of mercaptoethanol on the structure of the mitotic apparatus in sea urchin eggs.
Exp Cell Res. 1958 Aug;15(1):138-53 PMID: 13574167
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Exp Cell Res. 1960 Sep;20:529-47 PMID: 13788525

Article Info

Journal

The Journal of biophysical and biochemical cytology

Abbr.

J Biophys Biochem Cytol

ISSN

0095-9901

DOI

10.1083/jcb.10.4.467

Published

1961-08-00

Pages

467-74

Language

English

Region

United States

NLM ID

17840020R

PMCID

PMC2225108

Subset

External Links

PubMed source DOI: 10.1083/jcb.10.4.467 PMC Full Text

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The direct isolation of the mitotic apparatus.

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