Isolation of antibodies to gelatin from antigen-antibody complex by proteolysis.

Science (New York, N.Y.) ·Vol. 132 ·No. 3419 ·1960-07-08 ·Pages 86-7

ARNON R, SELA M

Abstract

Antibodies were isolated by digestion of the gelatin-antigelatin complex with collagenase and removal of the gelatin fragments by dialysis. The pure antibody could be precipitated anew by the antigen. Antibodies to tyrosylgelatin were isolated in a similar way, but in this case peptides containing the active antigenic sites remained bound to the antibody.

Keywords

ANTIBODIES

MeSH Terms

Antibodies Antigen-Antibody Complex Antigens Gelatin Humans Peptides Proteolysis

Chemicals

Antibodies Antigen-Antibody Complex Antigens Peptides Gelatin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

ARNON R

SELA M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.132.3419.86

Published

1960-07-08

Pages

86-7

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.132.3419.86 View journal details

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Isolation of antibodies to gelatin from antigen-antibody complex by proteolysis.

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