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PMID: 1379935 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of enterococcal isolates by restriction enzyme analysis of genomic DNA.

Epidemiology and infection ·Vol. 109 ·No. 1 ·1992-08-00 ·Pages 69-80

Lacoux PA, Jordens JZ, Fenton CM, Guiney M, Pennington TH

Abstract

A restriction enzyme analysis (REA) of chromosomal DNA for the intra-species characterization of enterococci is reported. The DNA was extracted by a rapid method and digested with the restriction enzyme Sal I to provide a characteristic 'fingerprint' consisting of 10-20 bands in the 1.6-5.0 kb range. One hundred and eighty enterococcal isolates were examined; 5 were type strains, 15 from an out-patient clinic and 160 from a geographically isolated British Antarctic Survey Base. The epidemiologically unrelated out-patient clinic isolates gave readily distinguishable patterns, whereas isolates from the geographically isolated community showed evidence of colonization. This technique provided a highly discriminatory method of isolate characterization for Enterococcus faecalis, E. faecium and E. durans suitable for epidemiological studies. A sample of isolates were probed with 16 + 23 S ribosomal RNA from Escherichia coli. Discrimination between isolates was poorer than with REA, although good correlation was observed between the results of the two techniques.

MeSH Terms

Antarctic Regions DNA Fingerprinting DNA, Bacterial/analysis Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Enterococcus/classification,genetics Enterococcus faecalis/classification Enterococcus faecium/classification Gram-Positive Bacterial Infections/microbiology Humans Lactococcus lactis/classification London Male Nucleic Acid Hybridization Prohibitins RNA, Bacterial/analysis RNA, Ribosomal/analysis Restriction Mapping Streptococcus bovis/classification

Chemicals

DNA, Bacterial PHB2 protein, human Prohibitins RNA, Bacterial RNA, Ribosomal Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific GTCGAC-specific type II deoxyribonucleases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lacoux P A

Department of Medical Microbiology, University of Aberdeen, Foresterhill, UK.

Jordens J Z

Fenton C M

Guiney M

Pennington T H

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18 references, click to expand

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Article Info

Journal

Epidemiology and infection

Abbr.

Epidemiol Infect

ISSN

0950-2688

Published

1992-08-00

Pages

69-80

Language

English

Region

England

NLM ID

8703737

PMCID

PMC2272234

Subset

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Characterization of enterococcal isolates by restriction enzyme analysis of genomic DNA.

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