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PMID: 13881912 Published · ppublish English Journal Article

Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture.

The Journal of general physiology ·Vol. 45 ·1962-01-00 ·Pages 439-85

COX RP, MACLEOD CM

Abstract

The alkaline phosphatase content of different tissue culture cell lines has been shown to vary from no detectable activity to high enzyme concentration. Within the epithelial lines studied alkaline phosphatase is either constitutive or inducible. Two epithelial cell strains in which alkaline phosphatase was "absent" could be induced to develop significant amounts of the enzyme when grown in the presence of Delta(1)-hydrocortisone. Phosphate did not repress enzyme induction by prednisolone. Under conditions of deadaptation the induced enzyme was diluted by cell multiplication. The mouse fibroblastic L line and several human fibroblastic lines did not contain alkaline phosphatase when grown under the conditions described nor could they be induced to produce the enzyme when cultivated in medium with prednisolone. Delta(1)-Hydrocortisone has other characteristic effects on established mammalian cell cultures which vary among cell lines. Human epithelial lines show reduction in cell multiplication with increase in mitotic index. The cytoplasm is increased and cell volume is nearly doubled. Mouse fibroblasts show a similar reduction in cell multiplication with a decrease in mitotic index. There is no increase in cell cytoplasm. Human fibroblast strains show no inhibition of multiplication or alteration in total cell protein when grown in medium containing prednisolone. Antisera prepared against "negative" prednisolone-inducible human cell lines and against a positive human line inhibited alkaline phosphatase activity to an equal degree.

Keywords
PHOSPHATASES/chemistry PREDNISOLONE/pharmacology TISSUE CULTURE
MeSH Terms
Alkaline Phosphatase Animals Cell Line Enzyme Induction Humans Mice Phosphates Phosphoric Monoester Hydrolases/chemistry Prednisolone/pharmacology Tissue Culture Techniques
Chemicals
Phosphates Prednisolone Alkaline Phosphatase Phosphoric Monoester Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
COX R P
MACLEOD C M
References (49)
49 references, click to expand
  1. Clonal growth in vitro of epithelial cells from normal human tissues.
    J Exp Med. 1956 Oct 1;104(4):615-28 PMID: 13367333
  2. Reduction of mitotic activity in pinna epidermis of mice given cortisol or 9alpha-fluorocortisol.
    Proc Soc Exp Biol Med. 1956 Apr;91(4):602-4 PMID: 13323016
  3. TISSUE CULTURE POPULATIONS AND THEIR RELATION TO THE TISSUE OF ORIGIN.
    Proc Natl Acad Sci U S A. 1960 Jul;46(7):963-72 PMID: 16590700
  4. Alkaline phosphatase in the meninges and in meningiomas.
    Nature. 1960 Jun 18;186:979 PMID: 14431667
  5. Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium (MB 752/1).
    J Natl Cancer Inst. 1959 May;22(5):1003-17 PMID: 13655076
  6. Effects of cortisone on regenerating rat liver.
    J Gen Physiol. 1954 Mar;37(4):559-74 PMID: 13143188
  7. Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells.
    Proc Soc Exp Biol Med. 1954 Feb;85(2):202-5 PMID: 13155547
  8. Serum alkaline phosphatase activity in diseases of the skeletal and hepatobiliary systems. A consideration of the current status.
    Am J Med. 1959 Dec;27:875-901 PMID: 13830463
  9. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
    Proc Soc Exp Biol Med. 1955 Jul;89(3):362-4 PMID: 13254761
  10. Growth and CF antigenicity of measles virus in cells deriving from human heart.
    Proc Soc Exp Biol Med. 1958 May;98(1):18-22 PMID: 13554528
  11. Action of adrenalcortical steroids on cultured cells.
    Endocrinology. 1959 Nov;65:777-84 PMID: 13829678
  12. A HeLa cell clonal line lacking alkaline phosphatase activity.
    Exp Cell Res. 1960 Dec;21:616-8 PMID: 13700894
  13. Chemical influences on cell division and development.
    Annu Rev Physiol. 1959;21:49-68 PMID: 13628007
  14. The inhibition by glutamine of glutamyl transferase formation in cultures of human cells.
    Biochim Biophys Acta. 1958 Feb;27(2):435-6 PMID: 13522759
  15. Effect of cortisone administration upon nucleic acid composition of rabbit liver.
    Proc Soc Exp Biol Med. 1951 Dec;78(3):818-24 PMID: 14912043
  16. Relation of oxygen and temperature in the preservation of tissues by refrigeration.
    Proc Soc Exp Biol Med. 1949 Jun;71(2):196-200 PMID: 18134009
  17. The effect of steroids on fibroblast migration in vitro. I. Cortisone, its inhibitory effect. II. 17-Hydroxycorticosterone, its inhibitory effect.
    Am J Pathol. 1953 Jul-Aug;29(4):761-6 PMID: 13065419
  18. Pharmacologic aspects of adrenocortical steroids and ACTH in man.
    N Engl J Med. 1953 Feb 5;248(6):232-45 PMID: 13025673
  19. The growth in vitro of single isolated tissue cells.
    J Natl Cancer Inst. 1948 Dec;9(3):229-46 PMID: 18105872
  20. Properties of a HeLa cell culture with increased resistance to poliomyelitis virus.
    Virology. 1958 Jun;5(3):425-34 PMID: 13557729
  21. The effect of massive doses of cortisone on the peripheral blood and bone marrow of the mouse.
    Blood. 1951 Jun;6(6):513-21 PMID: 14838962
  22. Effect of adrenocorticotrophic hormone on rheumatoid arthritis.
    Am J Med. 1949 Dec;7(6):741-50 PMID: 15394604
  23. Development of a chick embryo heart cell for the cultivation of poliovirus.
    Science. 1959 Apr 17;129(3355):1025-6 PMID: 13646637
  24. Immunization of humans with a chick embryo adapted strain of MEF1 poliomyelitis virus.
    J Immunol. 1956 Aug;77(2):123-31 PMID: 13357752
  25. Enzymes and cytological studies in rat hepatoma transplants, primary liver tumors, and in liver following azo dye feeding or partial hepatectomy.
    Cancer Res. 1957 Oct;17(9):862-79 PMID: 13472677
  26. ISOLATION AND STUDY OF MUTANTS FROM MAMMALIAN CELLS IN CULTURE.
    Proc Natl Acad Sci U S A. 1959 Jun;45(6):867-72 PMID: 16590461
  27. Eight culture strains (Detroit) of human epithelial-like cells.
    Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):730-5 PMID: 13370509
  28. Contributions of studies on the beta-galactosidase of Escherichia coli to our understanding of enzyme synthesis.
    Bacteriol Rev. 1957 Sep;21(3):140-68 PMID: 13471456
  29. Common antigens in tissue culture cell lines.
    Science. 1958 Jul 25;128(3317):198-9 PMID: 13568761
  30. Amino acid metabolism in mammalian cell cultures.
    Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
  31. Intracellular growth of pleuropneumonialike organisms (PPLO) in tissue culture and in ovo.
    Ann N Y Acad Sci. 1960 Jan 15;79:433-49 PMID: 14400338
  32. Continuous tissue culture of leukocytes from human leukemic bloods by application of gradient principles.
    Blood. 1955 Oct;10(10):1010-22 PMID: 13260360
  33. The establishment of strains of human cells in tissue culture.
    J Immunol. 1957 Jul;79(1):54-9 PMID: 13475808
  34. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.
    Biochim Biophys Acta. 1960 Mar 11;38:460-9 PMID: 13838951
  35. Nutrition of animal cells in tissue culture; initial studies on a synthetic medium.
    Proc Soc Exp Biol Med. 1950 Jan;73(1):1-8 PMID: 15402504
  36. Alkaline phosphatase in cell cultures of human origin.
    Nature. 1961 Mar 4;189:756-7 PMID: 13729282
  37. Culture characteristics of four permanent lines of human cancer cells.
    Cancer Res. 1955 Oct;15(9):598-602 PMID: 13261081
  38. Action of hydrocortisone on cells in tissue culture.
    Proc Soc Exp Biol Med. 1954 May;86(1):63-8 PMID: 13177591
  39. Pharmacologic aspects of adrenocortical steroids and ACTH in man.
    N Engl J Med. 1953 Apr 2;248(14):588-601; contd PMID: 13037002
  40. GENETICS OF SOMATIC MAMMALIAN CELLS : I. DEMONSTRATION OF THE EXISTENCE OF MUTANTS WITH DIFFERENT GROWTH REQUIREMENTS IN A HUMAN CANCER CELL STRAIN (HELA).
    J Exp Med. 1956 Sep 1;104(3):427-34 PMID: 19867118
  41. Continuous subcultivation of epithelial-like cells from normal human tissues.
    Proc Soc Exp Biol Med. 1954 Nov;87(2):440-3 PMID: 13237268
  42. The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure.
    J Exp Med. 1955 Nov 1;102(5):595-600 PMID: 13271674
  43. Studies on leukocyte alkaline phosphatase activity: relation to stress and pituitary-adrenal activity.
    J Lab Clin Med. 1954 Aug;44(2):219-28 PMID: 13184230
  44. Continuous cultivation of epithelial cell strain (FL) from human amniotic membrane.
    Proc Soc Exp Biol Med. 1957 Mar;94(3):532-7 PMID: 13408317
  45. Immunological studies with nucleoproteins from tissue-culture cells. II. Nucleoproteins from HeLa and H.Ep No. 1 cells.
    J Natl Cancer Inst. 1959 Sep;23:411-25 PMID: 13815377
  46. In vitro effects of cortisone on mesodermal tissue.
    AMA Arch Pathol. 1951 Jun;51(6):593-6 PMID: 14829131
  47. The establishment of a line (WISH) of human amnion cells in continuous cultivation.
    Exp Cell Res. 1961 Feb;23:14-20 PMID: 13712490
  48. [Studies on the determination of alkaline phosphatase in human cancer cells cultivated in vitro with special reference to their relationship to cell surfaces].
    Exp Cell Res. 1958 Aug;15(1):200-13 PMID: 13574173
  49. Effect of adrenal hormones and adrenalectomy on mitotic activity.
    Br J Exp Pathol. 1957 Feb;38(1):100-10 PMID: 13413086
Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.45.3.439
Published
1962-01-00
Pages
439-85
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2195181
Subset
OM
External Links
PubMed source DOI: 10.1085/jgp.45.3.439 PMC Full Text {# 免费全文（sci-hub.in）按钮暂时隐藏 Free Full Text #} View journal details
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