Electron transport particles from Bacillus stearothermophilus.

Downey, Ronald J. (University of Nebraska, Lincoln), Carl E. Georgi, and Walter E. Militzer. Electron transport particles from Bacillus stearothermophilus. J. Bacteriol. 83:1140-1146. 1962-Electron-transport particles (ETP) have been isolated from Bacillus stearothermophilus. They are capable of oxidizing such substrates as succinate, malate, diphosphopyridine nucleotide, p-phenylenediamine, and hydroquinone. Difference spectra indicated bands of cytochromes a(3), b, and c. A chromatographic procedure for purification of cytochrome c has been described. The role of quinonelike intermediates in the ETP was implied by the restorative effect of coenzyme Q and vitamin K(1) on material treated with lipid solvent. Isolation and identification of coenzyme Q from the thermophile indicated it was similar to coenzyme Q(10) of mammalian sources.