Interactions between the effects of alpha- and beta-adrenoceptor agonists and adenine nucleotides on the membrane potential of cells in guinea-pig liver slices.

British journal of pharmacology ·Vol. 59 ·No. 1 ·1977-01-00 ·Pages 163-75

Jenkinson DH, Koller K

Abstract

1 The beta-adrenoceptor agonist isoprenaline normally causes only a small and inconsistent increase in the membrane potential of cells in guinea-pig liver slices, in contrast to the large hyperpolarizations seen with alpha-agonists. However, after a selective alpha-adrenoceptor agonist has been applied, the response to isoprenaline becomes greatly enhanced. 2 Simultaneous application of small doses of an alpha- and beta-agonist produce hyperpolarizations larger than the sum of the responses to each agent alone. 3 These interactions occur with a range of sympathomimetic amines, including some which are not substrates for various processes for the uptake and inactivation of catecholamines. 4 Hyperpolarizations caused by externally applied cyclic adenosine-3',5'-monophosphate (cyclic AMP) also become larger after application of an alpha-agonist. 5 The adenine nucleotides adenosine 5'-diphosphate (ADP) and adenosine 5'-triphosphate (ATP) hyperpolarize guinea-pig liver cells in the dose range 0.1-1.0 mM. This response is not increased after an alpha-agonist. However, ADP and ATP are themselves able to enhance the response to beta-agonists. 6 These interactions between alpha-agonists, beta-agonists and adenine nucleotides seem to involve steps subsequent to receptor activation. Changes in the intracellular actions of cyclic AMP may be concerned.

MeSH Terms

Adenosine Diphosphate/pharmacology Adenosine Triphosphate/pharmacology Adrenergic alpha-Agonists/pharmacology Adrenergic beta-Agonists/pharmacology Albuterol/pharmacology Animals Cell Membrane/drug effects,physiology Cyclic AMP/pharmacology Dose-Response Relationship, Drug Guinea Pigs In Vitro Techniques Isoproterenol/pharmacology Liver/drug effects,physiology Male Membrane Potentials/drug effects Papaverine/pharmacology Phentolamine/pharmacology Phenylephrine/pharmacology

Chemicals

Adrenergic alpha-Agonists Adrenergic beta-Agonists Phenylephrine Adenosine Diphosphate Adenosine Triphosphate Papaverine Cyclic AMP Isoproterenol Albuterol Phentolamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jenkinson D H

Koller K

References (33)

33 references, click to expand

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Article Info

Journal

British journal of pharmacology

Abbr.

Br J Pharmacol

ISSN

0007-1188

DOI

10.1111/j.1476-5381.1977.tb06991.x

Published

1977-01-00

Pages

163-75

Language

English

Region

England

NLM ID

7502536

PMCID

PMC1667721

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1977.tb06991.x PMC Full Text View journal details

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Interactions between the effects of alpha- and beta-adrenoceptor agonists and adenine nucleotides on the membrane potential of cells in guinea-pig liver slices.

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