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PMID: 1394319 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the exopolysaccharide produced by Lactococcus lactis subspecies cremoris H414 grown in a defined medium or skimmed milk.

Carbohydrate research ·Vol. 231 ·1992-07-02 ·Pages 273-91

Gruter M, Leeflang BR, Kuiper J, Kamerling JP, Vliegenthart JF

Abstract

The structure of the exopolysaccharide of Lactococcus lactis subsp. cremoris H414, isolated from a defined medium or skimmed milk, was established by linkage analysis on the native polysaccharide, and by characterisation of oligosaccharide fragments, obtained by Smith degradation and partial acid hydrolysis, using methylation analysis, FABMS, EIMS, and 1H-NMR spectroscopy. The polysaccharide has the branched-pentasaccharide repeating unit: [formula: see text]

MeSH Terms
Animals Carbohydrate Conformation Carbohydrate Sequence Cattle Chromatography, High Pressure Liquid Culture Media Indicators and Reagents Lactococcus lactis/chemistry,growth & development,metabolism Magnetic Resonance Spectroscopy Mass Spectrometry Methylation Milk Molecular Sequence Data Oligosaccharides/chemistry,isolation & purification Polysaccharides, Bacterial/biosynthesis,chemistry,isolation & purification Spectrometry, Mass, Fast Atom Bombardment
Chemicals
Culture Media Indicators and Reagents Oligosaccharides Polysaccharides, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gruter M
Department of Bio-Organic Chemistry, Utrecht University, Netherlands.
Leeflang B R
Kuiper J
Kamerling J P
Vliegenthart J F
Article Info
Journal
Carbohydrate research
Abbr.
Carbohydr Res
ISSN
0008-6215
DOI
10.1016/0008-6215(92)84025-n
Published
1992-07-02
Pages
273-91
Language
English
Region
Netherlands
NLM ID
0043535
Subset
IM
External Links
PubMed source DOI: 10.1016/0008-6215(92)84025-n View journal details
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