Antigenicity of some Brucella melitensis cell fractions.
Glenchur, Harry (Minneapolis Veterans Administration Hospital, and University of Minnesota School of Medicine, Minneapolis), Ulysses S. Seal, Horace H. Zinneman, and Wendell H. Hall. Antigenicity of some Brucella melitensis cell fractions. J. Bacteriol. 85:363-368. 1963.-The components of a Brucella melitensis strain were obtained by differential centrifugation and diethylaminoethyl-cellulose ion-exchange chromatography. Rabbit antisera to these Brucella fractions were tested for agglutinins, precipitins, and blocking phenomena. Dermal hypersensitivity was also determined in rabbits. Insoluble (cell-wall) fractions provoked agglutinins, blocking antibodies and skin sensitivity, and precipitins for soluble antigens. Most soluble antigen fractions were capable of producing all the secondary immunological phenomena.
10 references, click to expand
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