Studies on L forms and protoplasts of group A streptococci. II. Chemical and immunological properties of the cell membrane.
Intact bacterial membranes have been isolated from protoplasts prepared from Group A streptococci by a cell wall-dissolving enzyme. A membrane fraction with identical chemical and serological properties has been obtained by differential centrifugation of mechanically disrupted streptococci. The membrane is chemically distinct from the cell wall and is composed of 72 per cent protein, 26 per cent lipid, and 2 per cent carbohydrate. Capillary precipitin tests and analysis by microdiffusion have demonstrated that the membrane contains antigens distinct from those of the cell wall and from those of the cytoplasm which it envelops. Evidence is presented which demonstrates that this antigenic material is common to the membranes of Group A streptococci, and that it can be distinguished by immunodiffusion from related antigenic substances present in membranes of several other serological groups of hemolytic streptococci. This antigenic material does not cross-react with the membrane antigens of other Gram-positive cocci.
18 references, click to expand
-
The glycerol-phospho-protein complex envelope of Micrococcus pyogenes.J Gen Microbiol. 1951 Nov;5(5 Suppl.):981-92 PMID: 14908035
-
Protoplasts of group A beta-haemolytic streptococci.Nature. 1958 Sep 20;182(4638):808-9 PMID: 13590125
-
The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. II. Nature of the cellular substrate attacked by the lytic enzymes.J Exp Med. 1952 Dec;96(6):569-80 PMID: 13022851
-
Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.J Exp Med. 1958 Dec 1;108(6):803-21 PMID: 13598814
-
Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.J Exp Med. 1961 Jul 1;114:127-40 PMID: 13754097
-
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
-
Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls.Biochim Biophys Acta. 1951 Jul;7(2):177-97 PMID: 14858403
-
Further studies on the chemical basis for serological specificity of Group A streptococcal carbohydrate.J Exp Med. 1958 Sep 1;108(3):311-23 PMID: 13575668
-
The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character.J Gen Microbiol. 1956 Jul;14(3):583-600 PMID: 13346020
-
Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety.J Exp Med. 1962 Jan 1;115:49-62 PMID: 14459433
-
Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of the carbohydrates.J Exp Med. 1956 Nov 1;104(5):629-43 PMID: 13367334
-
Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.Proc Natl Acad Sci U S A. 1962 Jan 15;48:41-9 PMID: 13878126
-
The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme.J Bacteriol. 1953 Dec;66(6):688-95 PMID: 13117795
-
[A micro-method of immuno-electrophoresis].Int Arch Allergy Appl Immunol. 1955;7(2):103-10 PMID: 13262900
-
The determination of glucosamine and galactosamine.Biochem J. 1955 Dec;61(4):586-9 PMID: 13276341
-
A simple method for the isolation and purification of total lipides from animal tissues.J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
-
TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES.J Exp Med. 1943 Aug 1;78(2):127-33 PMID: 19871314
-
Studies of L forms and protoplasts of group A streptococci. I. Isolation, growth, and bacteriologic characteristics.J Exp Med. 1959 Dec 1;110:853-74 PMID: 13824817
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
