Home LiteratureArticle Details
PMID: 1396551 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Heavy metal tolerance in the fission yeast requires an ATP-binding cassette-type vacuolar membrane transporter.

The EMBO journal ·Vol. 11 ·No. 10 ·1992-10-00 ·Pages 3491-9

Ortiz DF, Kreppel L, Speiser DM, Scheel G, McDonald G, Ow DW

Abstract

In response to heavy metal stress, plants and certain fungi, such as the fission yeast Schizosaccharomyces pombe, synthesize small metal-binding peptides known as phytochelatins. We have identified a cadmium sensitive S. pombe mutant deficient in the accumulation of a sulfide-containing phytochelatin-cadmium complex, and have isolated the gene, designated hmt1, that complements this mutant. The deduced protein sequence of the hmt1 gene product shares sequence identity with the family of ABC (ATP-binding cassette)-type transport proteins which includes the mammalian P-glycoproteins and CFTR, suggesting that the encoded product is an integral membrane protein. Analysis of fractionated fission yeast cell components indicates that the HMT1 polypeptide is associated with the vacuolar membrane. Additionally, fission yeast strains harboring an hmt1-expressing multicopy plasmid exhibit enhanced metal tolerance along with a higher intracellular level of cadmium, implying a relationship between HMT1 mediated transport and compartmentalization of heavy metals. This suggests that tissue-specific overproduction of a functional hmt1 product in transgenic plants might be a means to alter the tissue localization of these elements, such as for sequestering heavy metals away from consumable parts of crop plants.

MeSH Terms
ATP-Binding Cassette Transporters Adenosine Triphosphate/metabolism Amino Acid Sequence Base Sequence Cadmium/pharmacology Carrier Proteins/genetics,metabolism Chromosome Mapping Chromosomes, Fungal Cloning, Molecular DNA, Fungal/genetics Genes, Fungal Humans Intracellular Membranes/metabolism Intracellular Signaling Peptides and Proteins Membrane Proteins/genetics,metabolism Methyltransferases Molecular Sequence Data Open Reading Frames Protein-Arginine N-Methyltransferases Schizosaccharomyces/drug effects,genetics,metabolism Sequence Homology, Amino Acid Vacuoles/metabolism
Chemicals
ATP-Binding Cassette Transporters Carrier Proteins DNA, Fungal Intracellular Signaling Peptides and Proteins Membrane Proteins hmt1 protein, S pombe Cadmium Adenosine Triphosphate Methyltransferases PRMT2 protein, human Protein-Arginine N-Methyltransferases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ortiz D F
Plant Gene Expression Center, US Department of Agriculture, Albany, CA 94710.
Kreppel L
Speiser D M
Scheel G
McDonald G
Ow D W
References (40)
40 references, click to expand
  1. What determines the substrate specificity of the multi-drug-resistance pump?
    Trends Biochem Sci. 1990 Feb;15(2):42-6 PMID: 1970910
  2. Methods for studying the yeast vacuole.
    Methods Enzymol. 1991;194:644-61 PMID: 1706462
  3. Phytochelatins.
    Annu Rev Biochem. 1990;59:61-86 PMID: 2197985
  4. Improved method for the isolation of RNA from plant tissues.
    Anal Biochem. 1987 May 15;163(1):16-20 PMID: 2441623
  5. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.
    Science. 1989 Sep 8;245(4922):1066-73 PMID: 2475911
  6. P-glycoprotein: multidrug-resistance and a superfamily of membrane-associated transport proteins.
    FASEB J. 1989 Dec;3(14):2583-92 PMID: 2574119
  7. Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells.
    EMBO J. 1989 Dec 20;8(13):3973-84 PMID: 2686977
  8. Purification of vacuolar membranes, mitochondria, and plasma membranes from Neurospora crassa and modes of discriminating among the different H+-ATPases.
    Methods Enzymol. 1988;157:562-73 PMID: 2906719
  9. Quantitative assessment of worldwide contamination of air, water and soils by trace metals.
    Nature. 1988 May 12;333(6169):134-9 PMID: 3285219
  10. Sulfide stabilization of the cadmium-gamma-glutamyl peptide complex of Schizosaccharomyces pombe.
    J Biol Chem. 1988 Sep 15;263(26):12832-5 PMID: 3417637
  11. The detection and classification of membrane-spanning proteins.
    Biochim Biophys Acta. 1985 May 28;815(3):468-76 PMID: 3838905
  12. Signal sequences. The limits of variation.
    J Mol Biol. 1985 Jul 5;184(1):99-105 PMID: 4032478
  13. Analysis of membrane and surface protein sequences with the hydrophobic moment plot.
    J Mol Biol. 1984 Oct 15;179(1):125-42 PMID: 6502707
  14. Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase).
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6838-42 PMID: 16594069
  15. Cadmium-Sulfide Crystallites in Cd-(gammaEC)(n)G Peptide Complexes from Tomato.
    Plant Physiol. 1992 Jan;98(1):225-9 PMID: 16668618
  16. A P-glycoprotein homologue of Plasmodium falciparum is localized on the digestive vacuole.
    J Cell Biol. 1991 Jun;113(5):1033-42 PMID: 1674943
  17. Reactivation of metal-requiring apoenzymes by phytochelatin-metal complexes.
    FEBS Lett. 1991 Jun 17;284(1):66-9 PMID: 1905645
  18. The 70-kDa peroxisomal membrane protein is a member of the Mdr (P-glycoprotein)-related ATP-binding protein superfamily.
    J Biol Chem. 1990 Mar 15;265(8):4534-40 PMID: 1968461
  19. Tackling the protease problem in Saccharomyces cerevisiae.
    Methods Enzymol. 1991;194:428-53 PMID: 2005802
  20. A new class of lysosomal/vacuolar protein sorting signals.
    J Biol Chem. 1990 Apr 5;265(10):5349-52 PMID: 2180925
  21. The fungal vacuole: composition, function, and biogenesis.
    Microbiol Rev. 1990 Sep;54(3):266-92 PMID: 2215422
  22. Sequences encoded in the class II region of the MHC related to the 'ABC' superfamily of transporters.
    Nature. 1990 Dec 20-27;348(6303):741-4 PMID: 2259383
  23. Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle.
    J Cell Biol. 1987 Oct;105(4):1539-47 PMID: 2444598
  24. Expression of a multidrug resistance-adenosine deaminase fusion gene.
    J Biol Chem. 1989 May 5;264(13):7418-24 PMID: 2565338
  25. The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein.
    Nature. 1989 Aug 3;340(6232):400-4 PMID: 2569166
  26. Glutathione S-transferases: gene structure, regulation, and biological function.
    Annu Rev Biochem. 1989;58:743-64 PMID: 2673020
  27. Isolation of mutants of Schizosaccharomyces pombe unable to synthesize cadystin, small cadmium-binding peptides.
    Biochem Biophys Res Commun. 1988 Feb 29;151(1):32-9 PMID: 2894829
  28. Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa and Saccharomyces cerevisiae.
    Microbiol Rev. 1986 Sep;50(3):280-313 PMID: 2945985
  29. Nucleotide sequence of the structural genes for an anion pump. The plasmid-encoded arsenical resistance operon.
    J Biol Chem. 1986 Nov 15;261(32):15030-8 PMID: 3021763
  30. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.
    Nucleic Acids Res. 1987 Jun 11;15(11):4481-9 PMID: 3295780
  31. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  32. Brassica juncea Produces a Phytochelatin-Cadmium-Sulfide Complex.
    Plant Physiol. 1992 Jul;99(3):817-21 PMID: 16669006
  33. Metallothionein.
    Annu Rev Biochem. 1986;55:913-51 PMID: 3527054
  34. Accumulation of non-protein metal-binding polypeptides (gamma-glutamyl-cysteinyl)n-glycine in selected cadmium-resistant tomato cells.
    J Biol Chem. 1986 Oct 25;261(30):13879-82 PMID: 3771509
  35. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  36. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  37. Occurrence of acid-labile sulfide in cadmium-binding peptide 1 from fission yeast.
    J Biochem. 1983 Feb;93(2):661-4 PMID: 6841362
  38. Purification and unique properties in UV and CD spectra of Cd-binding peptide 1 from Schizosaccharomyces pombe.
    Biochem Biophys Res Commun. 1981 Dec 15;103(3):1021-8 PMID: 7332570
  39. Subcellular localization of cadmium and cadmium-binding peptides in tobacco leaves : implication of a transport function for cadmium-binding peptides.
    Plant Physiol. 1990 Apr;92(4):1086-93 PMID: 16667375
  40. Two pathways in the biosynthesis of cadystins (gamma EC)nG in the cell-free system of the fission yeast.
    Biochem Cell Biol. 1991 Feb-Mar;69(2-3):115-21 PMID: 2031714
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-10-00
Pages
3491-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556806
Subset
IM
Databases
GENBANK
S43129, X66358, X66359, X66360, X66361, X66362, X66363, X66364, X66365, Z14055
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2