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PMID: 1398113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human ribosomal protein S3a: cloning of the cDNA and primary structure of the protein.

Gene ·Vol. 119 ·No. 2 ·1992-10-01 ·Pages 313-6

Metspalu A, Rebane A, Hoth S, Pooga M, Stahl J, Kruppa J

Abstract

The amino acid (aa) sequence of human ribosomal protein S3a (hRPS3a) was deduced partially from the nucleotide sequence of the corresponding cDNA and confirmed by direct aa sequencing from the N terminus of the purified hRPS3a protein. The cDNA clone was isolated from a cDNA expression library in the pEX vector using antibodies. The hRPS3a protein has 263 aa and its calculated M(r) is 29 813.

Related Genes
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA HeLa Cells Humans Molecular Sequence Data RNA, Messenger/genetics Ribosomal Proteins/genetics
Chemicals
RNA, Messenger RPS3A protein, human Ribosomal Proteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Metspalu A
Estonian Biocenter, Tartu.
Rebane A
Hoth S
Pooga M
Stahl J
Kruppa J
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
Published
1992-10-01
Pages
313-6
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
Databases
GENBANK
M77234
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