A plaque method for rubella virus assay.

Canadian Medical Association journal ·Vol. 89 ·1963-08-17 ·Pages 314-5

ROZEE KR, GIVAN KF, KOANE FW, RHODES AJ

Abstract

A method has been described in which suitable dilutions of rubella virus will induce the formation, in a monolayer of green monkey kidney cells, of islets of infected cells which were protected from the effects of Echo 11 challenge virus. The number of islets or "negative" plaques was proportional to the dilution of rubella virus inoculated on to the monolayer. Using this method, it was observed that bentonite adsorption increased the plaque assay values of rubella virus pools. This suggested that rubella virus interference may be mediated by an interferon-like principle.

Keywords

ANIMAL VIRUSES RUBELLA

MeSH Terms

Animals Biological Assay Chlorocebus aethiops Enterovirus B, Human Interferons Rubella Rubella virus Viruses

Chemicals

Interferons

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

ROZEE K R

GIVAN K F

KOANE F W

RHODES A J

References (4)

4 references, click to expand

Rubella virus.
JAMA. 1962 Nov 10;182:663-71 PMID: 13988225
Association of virus with cases of rubella studied in Toronto: propagation of the agent and transmission to monkeys.
Can Med Assoc J. 1963 Jan 19;88:128-32 PMID: 13989076
Isolation in tissue culture of an interfering agent from patients with rubella.
Proc Soc Exp Biol Med. 1962 Nov;111:472-6 PMID: 13996865
VIRAL INTERFERENCE. SOME CONSIDERATIONS OF BASIC MECHANISMS AND THEIR POTENTIAL RELATIONSHIP TO HOST RESISTANCE.
Bacteriol Rev. 1960 Mar;24(1):151-66 PMID: 16350163

Article Info

Journal

Canadian Medical Association journal

Abbr.

Can Med Assoc J

ISSN

0008-4409

Published

1963-08-17

Pages

314-5

Language

English

Region

Canada

NLM ID

0414110

PMCID

PMC1921763

Subset

External Links

PubMed source PMC Full Text

Analysis Services

A plaque method for rubella virus assay.

Contact

WeChat Official Account