Characterization of ribosomes from Neurospora crassa.

Biophysical journal ·Vol. 3 ·1963-01-00 ·Pages 1-10

STORCK R

Abstract

Ribosomes isolated from growing hyphae of Neurospora crassa contain 53 per cent protein and 47 per cent RNA and have a sedimentation coefficient of 81S at 20 degrees C and infinite dilution. These ribosomes are stable at pH 7.4 in the presence of 0.01 M and 0.002 M MgCl(2) but undergo a dissociation into smaller particles if the MgCl(2) concentration is lowered to 0.0001 M. Two types of RNA with sedimentation coefficients of 19S(20) (50) and 13S(20) (50) have been extracted from the 81S particles.

Keywords

NUCLEOPROTEINS

MeSH Terms

Neurospora crassa Nucleoproteins Proteins RNA Ribosomes

Chemicals

Nucleoproteins Proteins RNA

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

STORCK R

References (17)

17 references, click to expand

Intracellular localization of inorganic polyphosphate in Neurospora crassa.
Biochim Biophys Acta. 1961 Jun 24;50:261-70 PMID: 13711668
A naturally occurring DNA-RNA complex from Neurospora crassa.
Proc Natl Acad Sci U S A. 1962 Jan 15;48:53-63 PMID: 13909469
The identification of a ribosomal-bound beta-glucosidase.
Proc Natl Acad Sci U S A. 1961 Apr 15;47:489-97 PMID: 13755892
Dissociation of macromolecular ribonucleoprotein of yeast.
Arch Biochem Biophys. 1957 Aug;70(2):426-31 PMID: 13459398
RNA, ribosomes, and protein synthesis.
Cold Spring Harb Symp Quant Biol. 1961;26:101-10 PMID: 13872836
AMINO ACID INCORPORATION INTO PROTEINS BY ESCHERICHIA COLI RIBOSOMES.
Proc Natl Acad Sci U S A. 1960 Nov;46(11):1450-63 PMID: 16590771
SYNTHESIS OF NASCENT PROTEIN BY RIBOSOMES IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1959 Sep;45(9):1437-47 PMID: 16590524
Estimation of nucleic acids.
Methods Biochem Anal. 1954;1:287-305 PMID: 13193533
Changes in microsomal components accompanying cell differentiation of pea-seedling roots.
Biochem J. 1961 Nov;81:254-60 PMID: 14466226
THE OCCURRENCE OF NATURAL DNA-RNA COMPLEXES IN E. COLI INFECTED WITH T2.
Proc Natl Acad Sci U S A. 1961 Aug;47(8):1135-41 PMID: 16590863
The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides.
Proc Natl Acad Sci U S A. 1961 Oct 15;47:1588-602 PMID: 14479932
The isolation and characterization of a macro-molecular ribonucleoprotein from yeast.
Arch Biochem Biophys. 1956 Mar;61(1):220-30 PMID: 13292958
Changes in ribosome structure and function during synchronized cell division.
Cold Spring Harb Symp Quant Biol. 1961;26:159-62 PMID: 14487186
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Electron microscopy of centrifuged hyphae of Neurospora.
J Biophys Biochem Cytol. 1961 Mar;9:609-17 PMID: 13787788
Some properties of ribonucleic acid from yeast 80S particle; effect of magnesium ions.
J Biochem. 1961 Nov;50:377-85 PMID: 14468148
The growth phase in Neurospora corresponding to the logarithmic phase in unicellular organisms.
J Bacteriol. 1950 Sep;60(3):221-3 PMID: 14774340

Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/s0006-3495(63)86800-9

Published

1963-01-00

Pages

1-10

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1366420

Subset

External Links

PubMed source DOI: 10.1016/s0006-3495(63)86800-9 PMC Full Text View journal details

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Characterization of ribosomes from Neurospora crassa.

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