Home LiteratureArticle Details
PMID: 1398981 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, Pan 1, is a lipoprotein.

Infection and immunity ·Vol. 60 ·No. 11 ·1992-11-00 ·Pages 4704-8

Hoehn GT, Clark VL

Abstract

Pan 1 is an acidic outer membrane protein of Neisseria gonorrhoeae that is expressed only when gonococci are grown anaerobically. On silver-stained sodium dodecyl sulfate-polyacrylamide gels, Pan 1 migrates as an intense but diffuse 54-kDa protein. The deduced amino acid sequence of Pan 1 from the aniA (anaerobically induced protein) open reading frame reveals a lipoprotein consensus sequence, Ala-Leu-Ala-Ala-Cys, and a processed molecular mass of 39 kDa. Furthermore, there is strong homology at the N terminus and C terminus of Pan 1 to the termini of the gonococcal outer membrane lipoproteins Lip and Laz. [3H]palmitic acid labeling of gonococci grown under oxygen-limited conditions demonstrated specific incorporation of label into Pan 1, suggesting further that Pan 1 is a lipoprotein.

Related Genes
aniA
MeSH Terms
Amino Acid Sequence Antigens, Bacterial/chemistry Bacterial Outer Membrane Proteins/chemistry Lipoproteins/chemistry Molecular Sequence Data Neisseria gonorrhoeae/chemistry,immunology Palmitic Acid Palmitic Acids/metabolism Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Lipoproteins Palmitic Acids aniA protein, Neisseria gonorrhoeae Palmitic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hoehn G T
Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, New York 14642.
Clark V L
References (19)
19 references, click to expand
  1. Isolation and nucleotide sequence of the gene (aniA) encoding the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae.
    Infect Immun. 1992 Nov;60(11):4695-703 PMID: 1383156
  2. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin.
    J Biol Chem. 1980 Apr 25;255(8):3707-12 PMID: 6988430
  3. Characterization of the neisserial lipid-modified azurin bearing the H.8 epitope.
    Mol Microbiol. 1989 May;3(5):583-91 PMID: 2474741
  4. The virulence-associated gonococcal H.8 gene encodes 14 tandemly repeated pentapeptides.
    Mol Microbiol. 1989 Jan;3(1):49-55 PMID: 2497299
  5. Coordinate regulation and sensory transduction in the control of bacterial virulence.
    Science. 1989 Feb 17;243(4893):916-22 PMID: 2537530
  6. Extracellular pullulanase of Klebsiella pneumoniae is a lipoprotein.
    J Bacteriol. 1986 Jun;166(3):1083-8 PMID: 3519575
  7. Comparison of the lipoprotein gene among the enterobacteriaceae. DNA sequence of Morganella morganii lipoprotein gene and its expression in Escherichia coli.
    J Biol Chem. 1983 Jul 10;258(13):8139-45 PMID: 6305973
  8. Mechanism of signal peptide cleavage in the biosynthesis of the major lipoprotein of the Escherichia coli outer membrane.
    J Biol Chem. 1982 May 10;257(9):5177-82 PMID: 7040395
  9. Distribution of a protein antigenically related to the major anaerobically induced gonococcal outer membrane protein among other Neisseria species.
    Infect Immun. 1990 Dec;58(12):3929-33 PMID: 2123827
  10. Lipoproteins in bacteria.
    J Bioenerg Biomembr. 1990 Jun;22(3):451-71 PMID: 2202727
  11. Localization of a conserved epitope and an azurin-like domain in the H.8 protein of pathogenic Neisseria.
    Mol Microbiol. 1987 Sep;1(2):179-85 PMID: 2452958
  12. Conserved lipoproteins of pathogenic Neisseria species bearing the H.8 epitope: lipid-modified azurin and H.8 outer membrane protein.
    Clin Microbiol Rev. 1989 Apr;2 Suppl:S1-4 PMID: 2470496
  13. Conserved lipoprotein H.8 of pathogenic Neisseria consists entirely of pentapeptide repeats.
    Mol Microbiol. 1989 Jan;3(1):43-8 PMID: 2497298
  14. Protein I: structure, function, and genetics.
    Clin Microbiol Rev. 1989 Apr;2 Suppl:S41-8 PMID: 2497962
  15. Induction and repression of outer membrane proteins by anaerobic growth of Neisseria gonorrhoeae.
    Infect Immun. 1987 Jun;55(6):1359-64 PMID: 3106220
  16. Presence of antibodies to the major anaerobically induced gonococcal outer membrane protein in sera from patients with gonococcal infections.
    Microb Pathog. 1988 Nov;5(5):381-90 PMID: 3148817
  17. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae.
    Infect Immun. 1984 Oct;46(1):202-12 PMID: 6207109
  18. Anaerobic growth of Neisseria gonorrhoeae coupled to nitrite reduction.
    Infect Immun. 1984 Oct;46(1):176-81 PMID: 6434425
  19. Analyses of gonococcal H8 antigen. Surface location, inter- and intrastrain electrophoretic heterogeneity, and unusual two-dimensional electrophoretic characteristics.
    J Exp Med. 1985 Dec 1;162(6):2017-34 PMID: 2415662
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.11.4704-4708.1992
Published
1992-11-00
Pages
4704-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258221
Subset
IM
Grants
NIAID NIH HHS · AI11709 · United States
External Links
PubMed source DOI: 10.1128/iai.60.11.4704-4708.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2