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PMID: 1400157 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Melting during steady-state transcription of the rrnB P1 promoter in vivo and in vitro.

Journal of bacteriology ·Vol. 174 ·No. 19 ·1992-10-00 ·Pages 6071-5

Ohlsen KL, Gralla JD

Abstract

The rRNA rrnB P1 promoter was probed with the single-strand-selective reagent potassium permanganate during steady-state transcription in vitro and in vivo. In both cases, a weak but significant level of permanganate sensitivity was observed, which was not changed by treatment with rifampin. In contrast, static studies showed that rifampin strongly affects the very high level signal associated with polymerases that have used ATP and CTP as initiating nucleotides. We infer that the permanganate sensitivity associated with steady-state transcription is due to polymerases that have not yet used ATP and CTP. The slow and regulated step during rrnB P1 transcription may be the use of the initiating nucleotides to catalyze stable opening of the promoter DNA.

MeSH Terms
DNA, Single-Stranded DNA-Directed RNA Polymerases/metabolism Nucleic Acid Denaturation Potassium Permanganate/pharmacology Promoter Regions, Genetic/genetics RNA, Ribosomal/genetics Rifampin/pharmacology Transcription, Genetic/drug effects
Chemicals
DNA, Single-Stranded RNA, Ribosomal Potassium Permanganate DNA-Directed RNA Polymerases Rifampin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ohlsen K L
University of California, Los Angeles.
Gralla J D
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.19.6071-6075.1992
Published
1992-10-00
Pages
6071-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207672
Subset
IM
Grants
NIGMS NIH HHS · GM07185 · United States
NIGMS NIH HHS · GM35754 · United States
External Links
PubMed source DOI: 10.1128/jb.174.19.6071-6075.1992 PMC Full Text View journal details
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