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PMID: 1401032 Published · ppublish English Comparative Study Journal Article

Evaluation and optimization of a latex agglutination assay for detection of cholera toxin and Escherichia coli heat-labile toxin.

Journal of clinical microbiology ·Vol. 30 ·No. 9 ·1992-09-00 ·Pages 2518-20

Yam WC, Lung ML, Ng MH

Abstract

The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the corresponding enteric pathogens in Southeast Asia. By selection of the appropriate culture media, the sensitivity of the assay was improved from 90.6% (for the detection of heat-labile toxin) and 75% (for the detection of cholera toxin) to 100%, and the results were confirmed with bioassays and DNA hybridization assays for both clinical and environmental isolates.

MeSH Terms
Agglutination Tests/methods Bacterial Toxins/analysis,immunology Biological Assay Cholera Toxin/analysis,immunology Enterotoxins/analysis,immunology Escherichia coli Proteins Evaluation Studies as Topic Immunoassay/methods Microspheres Nucleic Acid Hybridization Sensitivity and Specificity
Chemicals
Bacterial Toxins Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli Cholera Toxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yam W C
Department of Microbiology, University of Hong Kong.
Lung M L
Ng M H
References (15)
15 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.9.2518-2520.1992
Published
1992-09-00
Pages
2518-20
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265542
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.9.2518-2520.1992 PMC Full Text View journal details
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