FIBRINOGEN CLOTTING AND FIBRINO-PEPTIDE FORMATION BY STAPHYLOCOAGULASE AND THE COAGULASE-REACTING FACTOR.

Journal of bacteriology ·Vol. 85 ·1963-03-00 ·Pages 628-35

DRUMMOND MC, TAGER M

Abstract

Drummond, Margaret C. (Emory University, Atlanta, Ga.) and Morris Tager. Fibrinogen clotting and fibrino-peptide formation by staphylocoagulase and the coagulase-reacting factor. J. Bacteriol. 85:628-635. 1963.-The mechanism of fibrinogen clotting by staphylocoagulase and the coagulase-reacting factor (CRF) was studied from the standpoint of the products of the reaction, and compared with thrombin clotting of fibrinogen. Both clotting principles effect clotting with the release of nonprotein nitrogenous material, which can be resolved into three peptides by Dowex-50 chromatography. On the basis of the sedimentation constants, electrophoretic mobilities, and end-group analyses of these peptides, it appears that the products of fibrinogen clotting by coagulase-CRF and by thrombin are similar.

Keywords

FIBRIN FIBRINOGEN HYDROLASES PEPTIDES THROMBIN

MeSH Terms

Coagulase Fibrin Fibrinogen Hydrolases Peptides Resins, Synthetic Thrombin

Chemicals

Coagulase Peptides Resins, Synthetic Dowex 50 Fibrin Fibrinogen Hydrolases Thrombin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

DRUMMOND M C

TAGER M

References (14)

14 references, click to expand

Studies on the nature and the purification of the coagulase-reacting factor and its relation to prothrombin.
J Exp Med. 1956 Nov 1;104(5):675-86 PMID: 13367337
Fibrino-peptide.
Biochem J. 1952 Oct;52(2):200-3 PMID: 13018207
Thrombin-induced formation of co-fibrin. I. Isolation, purification, and characterization of co-fibrin.
J Biol Chem. 1959 Jan;234(1):62-6 PMID: 13610893
The clotting of fibrinogen. I. The liberation of peptide material.
Biochim Biophys Acta. 1955 Dec;18(4):495-503 PMID: 13304031
The activation of staphylococcal free coagulase by plasma constituents and the hydrolysis of Nalpha-toluene-p-sulphonyl-L-arginine methyl ester (TAME) by activated coagulase.
Biochem J. 1959 Feb;71(2):348-55 PMID: 13628577
Thrombin-induced formation of co-fibrin. III. Acid degradation studies and summary of sequential evidence on peptide A.
J Biol Chem. 1959 Sep;234:2317-20 PMID: 13823737
The clotting of fibrinogen. II. Fractionation of peptide material liberated.
Biochim Biophys Acta. 1956 Jan;19(1):121-30 PMID: 13304078
The thrombin-induced formation of co-fibrin. II. Preliminary amino acid sequence studies on peptides A and B.
J Biol Chem. 1959 Jan;234(1):67-70 PMID: 13610894
Enzymatic activities associated with clotting of fibrinogen by staphylocoagulase and coagulase-reacting factor and their inhibition by disopropylfluophosphate.
J Bacteriol. 1962 May;83:975-80 PMID: 13887961
The action of thrombin on fibrinogen.
Biochem J. 1952 Oct;52(2):196-9 PMID: 13018206
Separation of acid phosphatase from tributyrinase and plasma clotting activities in partially purified staphylocoagulase.
J Bacteriol. 1962 Feb;83:432 PMID: 13887962
Enzymatic activity of staphylocoagulase. I. Characterization of an esterase associated with purified preparations.
J Bacteriol. 1959 Sep;78:407-12 PMID: 13818238
The polymerization of proteins; the action of thrombin on fibrinogen.
Arch Biochem Biophys. 1951 Jul;32(2):317-24 PMID: 14857750
The free amino groups of insulin.
Biochem J. 1945;39(5):507-15 PMID: 16747948

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.85.3.628-635.1963

Published

1963-03-00

Pages

628-35

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC278193

Subset

External Links

PubMed source DOI: 10.1128/jb.85.3.628-635.1963 PMC Full Text View journal details

Analysis Services

FIBRINOGEN CLOTTING AND FIBRINO-PEPTIDE FORMATION BY STAPHYLOCOAGULASE AND THE COAGULASE-REACTING FACTOR.

Contact

WeChat Official Account