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PMID: 1406594 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the imidazoleglycerolphosphate dehydratase gene of Trichoderma harzianum by genetic complementation in Saccharomyces cerevisiae using a direct expression vector.

Molecular & general genetics : MGG ·Vol. 234 ·No. 3 ·1992-09-00 ·Pages 481-8

Goldman GH, Demolder J, Dewaele S, Herrera-Estrella A, Geremia RA, Van Montagu M, Contreras R

Abstract

The Trichoderma harzianum imidazoleglycerolphosphate dehydratase gene (igh) has been isolated by complementation of a Saccharomyces cerevisiae his3 mutant using a direct expression vector. This Escherichia coli-yeast shuttle vector was developed to allow efficient cloning and expression of cDNA libraries. The cDNA is 627 nucleotides long and codes for a protein of 209 amino acids with an apparent molecular mass of 22,466 daltons. The predicted protein sequence showed 63.6%, 58.7%, and 38.4% identity respectively to the corresponding enzymes from S. cerevisiae, Pichia pastoris and E. coli. Northern analysis showed that the expression of the igh gene in T. harzianum is not inhibited by external histidine and the level of igh mRNA was about threefold higher in cells starved of histidine.

Related Genes
igh
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Fungal Proteins/genetics Gene Expression Genes, Fungal Genetic Complementation Test Genetic Vectors Hydro-Lyases/genetics Molecular Sequence Data RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics Trichoderma/genetics
Chemicals
Fungal Proteins RNA, Messenger Hydro-Lyases imidazoleglycerolphosphate dehydratase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Goldman G H
Laboratorium voor Genetica, Universiteit Gent, Belgium.
Demolder J
Dewaele S
Herrera-Estrella A
Geremia R A
Van Montagu M
Contreras R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00538709
Published
1992-09-00
Pages
481-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
Z11528
External Links
PubMed source DOI: 10.1007/BF00538709
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