STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES.

The Journal of cell biology ·Vol. 19 ·1963-10-00 ·Pages 59-69

BENDICH A, BORENFREUND E, HUTCHISON DJ

Abstract

A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells has been developed. Cells, arrested at metaphase with colchicine, were exposed to hypotonic solution and the pH was then adjusted to 5.6 to stabilize the chromosomes. The metaphase figures were subsequently disrupted and the chromosomes isolated by a series of differential centrifugations in sucrose. The isolated chromosomes were well preserved, as judged by morphological criteria. The effect of various enzymes and chemical agents on the isolated chromosomes was studied. Chymotrypsin, trypsin, and deoxyribonuclease caused a marked disintegration of the chromosomes, whereas treatment with pepsin and ribonuclease induced no significant morphological alterations.

Keywords

CENTRIFUGATION CHROMOSOMES CHYMOTRYPSIN COLCHICINE DEOXYRIBONUCLEASE EXPERIMENTAL LAB STUDY LEUKEMIA EXPERIMENTAL LYMPHOCYTES MICE PEPSIN PHARMACOLOGY RIBONUCLEASE TRYPSIN

MeSH Terms

Centrifugation Chromosomes Chymotrypsin Colchicine DNA Deoxyribonucleases Leukemia Leukemia, Experimental Lymphocytes Metaphase Mice Pepsin A Pharmacology Research Ribonucleases Trypsin

Chemicals

DNA Deoxyribonucleases Ribonucleases Chymotrypsin Trypsin Pepsin A Colchicine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

BENDICH A

BORENFREUND E

HUTCHISON D J

References (11)

11 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.19.1.59

Published

1963-10-00

Pages

59-69

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2106868

Subset

External Links

PubMed source DOI: 10.1083/jcb.19.1.59 PMC Full Text View journal details

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