THE ACTION OF ENZYMES FROM CLOSTRIDIUM TERTIUM ON THE I ANTIGENIC DETERMINANT OF HUMAN ERYTHROCYTES.

The Journal of experimental medicine ·Vol. 118 ·1963-08-01 ·Pages 175-94

MARCUS DM, KABAT EA, ROSENFIELD RE

Abstract

A method was described for the partial purification of beta galactosidase and beta glucosaminidase from Clostridium tertium culture supernatants. Treatment of erythrocytes with preparations containing both enzymes decreases their ability to react with anti-I cold agglutinins, and with Type XIV antipneumococcal horse serum. Erythrocytes of blood group A(1) are altered more rapidly and extensively than are group O cells. The enzymatic treatment of stroma results in a decrease in ability to absorb anti-I agglutinins and the release of galactose and N-acetylglucosamine as monosaccharides. The data suggest that these two sugars may be structural units of the erythrocyte I determinant, but no direct evidence is available.

Keywords

ANTIBODIES BLOOD GROUPS CLOSTRIDIUM ERYTHROCYTES GALACTOSIDASE GLUCOSAMINE GLYCOSIDE HYDROLASES HEMAGGLUTINATION

MeSH Terms

Antibodies Blood Group Antigens Clostridium Clostridium tertium Epitopes Erythrocytes Galactose Galactosidases Glucosamine Glycoside Hydrolases Hemagglutination Hemagglutination Tests Humans

Chemicals

Antibodies Blood Group Antigens Epitopes Galactosidases Glycoside Hydrolases Glucosamine Galactose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

MARCUS D M

KABAT E A

ROSENFIELD R E

References (28)

28 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.118.2.175

Published

1963-08-01

Pages

175-94

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2137716

Subset

External Links

PubMed source DOI: 10.1084/jem.118.2.175 PMC Full Text View journal details

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THE ACTION OF ENZYMES FROM CLOSTRIDIUM TERTIUM ON THE I ANTIGENIC DETERMINANT OF HUMAN ERYTHROCYTES.

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