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PMID: 1409658 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 20 ·1992-10-15 ·Pages 9479-83

Verma R, Smiley J, Andrews B, Campbell JL

Abstract

In Saccharomyces cerevisiae, at least 17 DNA replication genes are coordinately expressed at the G1/S boundary during the cell cycle. All of these genes have the DNA sequence element ACGCGT in their 5' upstream regulatory regions. This sequence has been shown to be essential for periodic expression of the POL1, CDC9, and TMP1 genes. The cyclin (CLN1 and CLN2) and HO genes are another subset of genes that are expressed with the same timing as the DNA replication genes. Their periodic expression requires the participation of two well-characterized transcriptional activators: the SWI4 and SWI6 gene products. In this study, we present evidence that SWI6 contributes to the regulation of DNA replication genes as well. Surprisingly, a preferential requirement for SWI6 over SWI4 is observed in our studies of ACGCGT-dependent reporter gene expression in vivo. This selectivity has not been observed for the other G1/S genes. Correlating with the in vivo results, protein-DNA complexes formed in vitro on multimeric ACGCGT elements are either abolished or reduced in swi6 delta deletion mutants.

Related Genes
ACT POL1 SWI6
MeSH Terms
Base Sequence Cell Cycle DNA Replication DNA, Fungal/metabolism DNA-Binding Proteins/metabolism DNA-Directed DNA Polymerase/genetics Deoxyribonucleases, Type II Site-Specific Genes, Fungal Macromolecular Substances Mating Factor Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Peptides/physiology Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/cytology,genetics
Chemicals
DNA, Fungal DNA-Binding Proteins Macromolecular Substances Oligodeoxyribonucleotides Peptides Mating Factor DNA-Directed DNA Polymerase Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Verma R
Braun Laboratories, California Institute of Technology, Pasadena 91125.
Smiley J
Andrews B
Campbell J L
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.20.9479
Published
1992-10-15
Pages
9479-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50155
Subset
IM
Grants
NIGMS NIH HHS · GM25508 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.20.9479 PMC Full Text View journal details
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