A SENSITIVE METHOD FOR THE ASSAY OF CATECHOL AMINES.

British journal of pharmacology and chemotherapy ·Vol. 22 ·1964-02-00 ·Pages 204-10

ARMITAGE AK, VANE JR

Abstract

A strip of fundus from a rat's stomach was suspended in Krebs solution containing 5-hydroxytryptamine. Movements of the muscle were recorded by means of a frontal writing lever giving a magnification of sixteen-times. The strip relaxed when isoprenaline, adrenaline or noradrenaline was added to the organ-bath in concentrations of 0.2 to 2 ng/ml. The preparation was most sensitive to isoprenaline and least sensitive to noradrenaline. The components of a mixture of two catechol amines could be assayed by superfusing the rat stomach and a chick rectum in the same stream of fluid.

Keywords

BIOLOGICAL ASSAY EPINEPHRINE EXPERIMENTAL LAB STUDY ISOPROTERENOL NOREPINEPHRINE PHARMACOLOGY POULTRY RATS RECTUM STOMACH

MeSH Terms

Amines Animals Biological Assay Catechols Epinephrine Female Humans Isoproterenol Muscles Norepinephrine Pharmacology Poultry Rats Rectum Research Serotonin Stomach Uterus

Chemicals

Amines Catechols Serotonin Isoproterenol catechol Norepinephrine Epinephrine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

ARMITAGE A K

VANE J R

References (8)

8 references, click to expand

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Br J Pharmacol Chemother. 1949 Dec;4(4):401-8 PMID: 15398552
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Article Info

Journal

British journal of pharmacology and chemotherapy

Abbr.

Br J Pharmacol Chemother

ISSN

0366-0826

DOI

10.1111/j.1476-5381.1964.tb01560.x

Published

1964-02-00

Pages

204-10

Language

English

Region

England

NLM ID

0154627

PMCID

PMC1703914

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1964.tb01560.x PMC Full Text

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