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PMID: 1416843 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Innovative endpoint determination system for antifungal susceptibility testing of yeasts.

Antimicrobial agents and chemotherapy ·Vol. 36 ·No. 8 ·1992-08-00 ·Pages 1619-25

Tellier R, Krajden M, Grigoriew GA, Campbell I

Abstract

Fungal infections and antifungal resistance are increasingly recognized. Antifungal susceptibility testing remains unstandardized, and a particularly important problem is endpoint determination. In this paper we propose the yeast metabolic reduction of the tetrazolium salt 2,3-bis(2- methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetraz olium hydroxide (XTT) as a colorimetric endpoint which is quantitative and objective. Amphotericin B, fluorocytosine, and fluconazole dose-response curves were obtained, and a metabolic MIC could be determined by using precise criteria.

MeSH Terms
Antifungal Agents/pharmacology Candida/drug effects Candida albicans/drug effects Colorimetry Culture Media Indicators and Reagents Microbial Sensitivity Tests/standards Oxidation-Reduction Tetrazolium Salts Yeasts/drug effects
Chemicals
Antifungal Agents Culture Media Indicators and Reagents Tetrazolium Salts 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tellier R
Department of Microbiology, Toronto Hospital, Ontario, Canada.
Krajden M
Grigoriew G A
Campbell I
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16 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.36.8.1619
Published
1992-08-00
Pages
1619-25
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC192019
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.36.8.1619 PMC Full Text View journal details
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