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PMID: 14176290 Published · ppublish English Journal Article

THE REQUIREMENT FOR HYDROCORTISONE IN ANTIBODY-FORMING TISSUE CULTIVATED IN SERUM-FREE MEDIUM.

The Journal of experimental medicine ·Vol. 119 ·1964-01-01 ·Pages 1027-49

AMBROSE CT

Abstract

It was previously reported from this laboratory that the secondary antibody response can regularly be elicited in vitro from fragments of rabbit lymph node node cultured in Eagle's medium supplemented with normal rabbit serum. Evidence is now presented that physiological levels of hydrocortisone (0.01 to 1.0 microM) can substitute for serum in the culture medium. However, with the omission of serum, serine (0.1 mM) must be included among Eagle's "essential" amino acids for consistent optimal antibody production. In some experiments the addition of insulin (0.5 unit/ml) and vitamin B(12) (0.5 microg/ml) has further enhanced the secondary response in this serum-free medium.

Keywords
ADRENAL CORTEX HORMONES AMINO ACIDS ANTIBODY FORMATION CULTURE MEDIA EXPERIMENTAL LAB STUDY HYDROCORTISONE INSULIN RABBITS SUCCINATES TISSUE CULTURE TRIIODOTHYRONINE VITAMIN B 12 VITAMINS
MeSH Terms
Adrenal Cortex Hormones Amino Acids Animals Antibody Formation Culture Media Hydrocortisone Insulin Rabbits Research Succinates Tissue Culture Techniques Triiodothyronine Vitamin A Vitamin B 12 Vitamins
Chemicals
Adrenal Cortex Hormones Amino Acids Culture Media Insulin Succinates Vitamins Triiodothyronine Vitamin A Vitamin B 12 Hydrocortisone
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
AMBROSE C T
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45 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.119.6.1027
Published
1964-01-01
Pages
1027-49
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2137758
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.119.6.1027 PMC Full Text View journal details
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