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PMID: 14188734 Published · ppublish English Journal Article

PLAQUE ASSAY FOR PSITTACOSIS VIRUS IN MONOLAYERS OF CHICK EMBRYO FIBROBLASTS.

Journal of bacteriology ·Vol. 87 ·1964-06-00 ·Pages 1503-11

PIRAINO F, ABEL C

Abstract

Piraino, F. (City of Milwaukee Health Department, Milwaukee, Wis.), and C. Abel. Plaque assay for psittacosis virus in monolayers of chick embryo fibroblasts. J. Bacteriol. 87:1503-1511. 1964.-A tissue culture system for the assay of 6BC psittacosis virus by plaque production on chick embryo fibroblast cells is described. Experiments are given which characterize the specificity of the reaction and identify factors found to cause variations in plaque counts. The statistical data obtained indicate this assay to be a precise and highly reliable procedure for the detection of 6BC virus and antibody.

Keywords
ANTIBODIES CHICK EMBRYO DIAGNOSIS EXPERIMENTAL LAB STUDY MIYAGAWANELLA ORNITHOSIS PENICILLIN PHARMACOLOGY STREPTOMYCIN TISSUE CULTURE VIRUS CULTIVATION
MeSH Terms
Antibodies Chick Embryo Chlamydia Chlamydophila psittaci Diagnosis Fibroblasts Penicillins Pharmacology Psittacosis Research Streptomycin Tissue Culture Techniques Virus Cultivation
Chemicals
Antibodies Penicillins Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
PIRAINO F
ABEL C
References (5)
5 references, click to expand
  1. An analysis of the assay of Rous sarcoma cells in vitro by the infective center technique.
    Virology. 1960 Jan;10:29-49 PMID: 14439880
  2. Growth of psittacosis virus in tissue culture.
    J Infect Dis. 1960 Nov-Dec;107:283-99 PMID: 13730378
  3. A plaque assay for meningopneumonitis virus in monolayers of strain L cells.
    Virology. 1960 Dec;12:578-88 PMID: 13714182
  4. A radiological study of cell-virus interaction in the Rous sarcoma.
    Virology. 1959 Jan;7(1):75-91 PMID: 13636057
  5. Serial changes in virus and cells in cultures chronically infected with psittacosis virus.
    Virology. 1961 May;14:88-99 PMID: 13730379
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1964-06-00
Pages
1503-11
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC277232
Subset
OM
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