TRANSFORMATION TO PROTOTROPHY AND POLYGLUTAMIC ACID SYNTHESIS IN BACILLUS LICHENIFORMIS.

Journal of bacteriology ·Vol. 88 ·1964-07-00 ·Pages 220-5

LEONARD CG, MATTHEIS DK, MATTHEIS MJ, HOUSEWRIGHT RD

Abstract

Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).

Keywords

BACILLUS EXPERIMENTAL LAB STUDY GENETICS GLUTAMATES METABOLISM MUTATION

MeSH Terms

Bacillus Genetics Glutamates Metabolism Mutation Polyglutamic Acid Research Serine

Chemicals

Glutamates Polyglutamic Acid Serine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

LEONARD C G

MATTHEIS D K

MATTHEIS M J

HOUSEWRIGHT R D

References (13)

13 references, click to expand

REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.
J Bacteriol. 1963 Sep;86:392-400 PMID: 14066414
Studies on transformations of Hemophilus influenzae. I. Competence.
J Gen Physiol. 1961 Jul;44:1201-27 PMID: 13707010
Transformability of Haemophilus influenzae.
J Gen Microbiol. 1962 Nov;29:537-49 PMID: 13979011
Transduction in Bacillus subtilis.
J Bacteriol. 1962 Jan;83:106-11 PMID: 13921030
Isolation of D- and L-glutamyl polypeptides from culture filtrates of Bacillus subtilis.
J Biol Chem. 1958 Nov;233(5):1109-12 PMID: 13598742
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
POLYGLUTAMIC ACID SYNTHESIS BY CELL-FREE EXTRACTS OF BACILLUS LICHENIFORMIS.
Biochim Biophys Acta. 1963 Jul 9;73:530-2 PMID: 14068536
CONCENTRATION AND ISOLATION OF AUXOTROPHIC MUTANTS OF SPOREFORMING BACTERIA.
J Bacteriol. 1960 Feb;79(2):309-10 PMID: 16561851
Effects of some metallic ions on glutamyl polypeptide synthesis by Bacillus subtilis.
J Bacteriol. 1958 Nov;76(5):499-503 PMID: 13598708
TRANSFORMATION OF BACILLUS LICHENIFORMIS.
J Bacteriol. 1964 Mar;87:519-26 PMID: 14127566
Molecular fate of DNA in genetic transformation of Pneumococcus.
J Mol Biol. 1962 Jul;5:119-31 PMID: 14461406
PENETRATION OF DEOXYRIBONUCLEIC ACID INTO HEMOPHILUS INFLUENZAE.
Biochim Biophys Acta. 1963 Sep 17;76:25-39 PMID: 14068558

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.88.1.220-225.1964

Published

1964-07-00

Pages

220-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC277280

Subset

External Links

PubMed source DOI: 10.1128/jb.88.1.220-225.1964 PMC Full Text View journal details

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