LEAF-NODULE SYMBIOSIS. I. ENDOPHYTE OF PSYCHOTRIA BACTERIOPHILA.

Centifanto, Ysolina M. (University of Florida, Gainesville), and Warren S. Silver. Leafnodule symbiosis. I. Endophyte of Psychotria bacteriophila. J. Bacteriol. 88:776-781. 1964.-The leaf-nodule endophyte of Psychotria bacteriophila has been repeatedly isolated in pure culture from germinating seedlings and young leaves on a nitrogen-free mineral agar. Its morphological, serological, and cultural characteristics place it within the Klebsiella-Aerobacter group. The endophyte has been provisionally named Klebsiella rubiacearum. Pure cultures fix N(2) under anaerobic conditions with good efficiency (4.54 mug of N fixed/mg of glucose utilized in 3 days). Nitrogen fixation by pure cultures is intimately related to pyruvate and hydrogen metabolism.